CRISPR-U™ Gene Knockout Cell Line Strategy

BRWD1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BRWD1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BRWD1
Gene id 54014
Organism Homo sapiens
Official full symbol bromodomain and WD repeat domain containing 1
Gene type protein-coding
Also known as C21orf107, DCAF19, N143, WDR9, WRD9
Summary This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD) residues which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 2 bromodomains and multiple WD repeats. This gene is located within the Down syndrome region-2 on chromosome 21. Alternative splicing of this gene generates multiple transcript variants encoding distinct isoforms. In mouse, this gene encodes a nuclear protein that has a polyglutamine-containing region that functions as a transcriptional activation domain which may regulate chromatin remodelling and associates with a component of the SWI/SNF chromatin remodelling complex.
Genomic regions Chromosome 21
Strategy Summary
This gene has 6 protein coding transcripts：
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BRWD1-203 ENST00000342449.8 17728 2269aa Protein coding CCDS13663 Q9NSI6-2 NM_033656.4 TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92,
BRWD1-201 ENST00000333229.6 10141 2320aa Protein coding CCDS13662 Q9NSI6-1 - TSL:1, GENCODE basic, APPRIS P4,
BRWD1-202 ENST00000341322.4 2495 120aa Protein coding CCDS33557 Q9NSI6-4 - TSL:1, GENCODE basic,
BRWD1-204 ENST00000380800.7 7605 2199aa Protein coding - Q9NSI6-3 - TSL:1, GENCODE basic, APPRIS ALT2,
BRWD1-211 ENST00000455867.5 1644 548aa Protein coding - H0Y463 - CDS 5' and 3' incomplete, TSL:1,
BRWD1-206 ENST00000424441.1 1356 452aa Protein coding - H7C409 - CDS 5' and 3' incomplete, TSL:1,
BRWD1-210 ENST00000446924.5 4990 640aa Nonsense mediated decay - H7BZR9 - CDS 5' incomplete, TSL:2,
BRWD1-205 ENST00000412604.1 1756 467aa Nonsense mediated decay - H0Y5I9 - CDS 5' incomplete, TSL:1,
BRWD1-208 ENST00000445245.5 1682 235aa Nonsense mediated decay - H0Y4A7 - CDS 5' incomplete, TSL:1,
BRWD1-207 ENST00000430093.5 1656 266aa Nonsense mediated decay - H0Y4R9 - CDS 5' incomplete, TSL:1,
BRWD1-209 ENST00000445668.5 1593 409aa Nonsense mediated decay - H0Y500 - CDS 5' incomplete, TSL:1,
BRWD1-212 ENST00000470108.5 1176 No protein Processed transcript - - - TSL:3,
BRWD1-213 ENST00000473813.1 944 No protein Retained intron - - - TSL:3,
BRWD1-215 ENST00000491564.1 522 No protein Retained intron - - - TSL:1,
BRWD1-214 ENST00000484090.1 429 No protein Retained intron - - - TSL:2,
BRWD1-216 ENST00000496759.1 377 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BRWD1
This KO Strategy loading
Red Cotton™ Notes Gene BRWD1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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