CRISPR-U™ Gene Knockout Cell Line
Strategy
CSNK1G1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CSNK1G1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CSNK1G1 |
| Gene id | 53944 |
| Organism | Homo sapiens |
| Official full symbol | casein kinase 1 gamma 1 |
| Gene type | protein-coding |
| Also known as | CK1gamma1 |
| Summary | This gene encodes a member of the casein kinase I gene family. This family is comprised of serine/threonine kinases that phosphorylate acidic proteins such as caseins. The encoded kinase plays a role in cell cycle checkpoint arrest in response to stalled replication forks by phosphorylating Claspin. A mutation in this gene may be associated with non-syndromic early-onset epilepsy (NSEOE). |
| Genomic regions | Chromosome 15 |
Strategy Summary
This gene has 14 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CSNK1G1-201 | ENST00000303052.13 | 8085 | 422aa | Protein coding | CCDS10192 | Q9HCP0-1 | NM_022048.5 | TSL:1, GENCODE basic, MANE Select v0.92, |
| CSNK1G1-205 | ENST00000607537.6 | 2083 | 475aa | Protein coding | CCDS86465 | U3KQB3 | - | TSL:1, GENCODE basic, |
| CSNK1G1-202 | ENST00000561349.6 | 1756 | 438aa | Protein coding | CCDS86464 | Q8IXA3 | - | TSL:1, GENCODE basic, |
| CSNK1G1-211 | ENST00000635142.1 | 5548 | 430aa | Protein coding | - | A0A0U1RQT7 | - | TSL:5, GENCODE basic, |
| CSNK1G1-209 | ENST00000634811.1 | 2592 | 459aa | Protein coding | - | A0A0U1RQY7 | - | TSL:5, GENCODE basic, APPRIS P1, |
| CSNK1G1-207 | ENST00000634654.1 | 2485 | 459aa | Protein coding | - | A0A0U1RQY7 | - | TSL:5, GENCODE basic, APPRIS P1, |
| CSNK1G1-204 | ENST00000606793.3 | 1658 | 422aa | Protein coding | - | U3KQA5 | - | TSL:5, GENCODE basic, |
| CSNK1G1-213 | ENST00000635270.1 | 830 | 183aa | Protein coding | - | A0A0U1RR14 | - | CDS 3' incomplete, TSL:5, |
| CSNK1G1-217 | ENST00000635514.1 | 790 | 138aa | Protein coding | - | A0A0U1RQK2 | - | CDS 3' incomplete, TSL:5, |
| CSNK1G1-206 | ENST00000634302.1 | 712 | 204aa | Protein coding | - | A0A0U1RQK6 | - | CDS 5' incomplete, TSL:5, |
| CSNK1G1-210 | ENST00000634909.1 | 615 | 173aa | Protein coding | - | A0A0U1RQG9 | - | CDS 5' incomplete, TSL:5, |
| CSNK1G1-208 | ENST00000634722.1 | 601 | 158aa | Protein coding | - | A0A0U1RR31 | - | CDS 5' incomplete, TSL:2, |
| CSNK1G1-216 | ENST00000635414.1 | 600 | 67aa | Protein coding | - | A0A0U1RQN5 | - | CDS 5' incomplete, TSL:3, |
| CSNK1G1-212 | ENST00000635230.1 | 463 | 36aa | Protein coding | - | A0A0U1RQU4 | - | CDS 5' incomplete, TSL:3, |
| CSNK1G1-203 | ENST00000606225.1 | 1950 | 393aa | Nonsense mediated decay | - | Q9HCP0-2 | - | TSL:1, |
| CSNK1G1-218 | ENST00000635529.1 | 814 | 77aa | Nonsense mediated decay | - | A0A0U1RRH5 | - | TSL:5, |
| CSNK1G1-214 | ENST00000635324.1 | 799 | 114aa | Nonsense mediated decay | - | A0A0U1RQH9 | - | TSL:5, |
| CSNK1G1-215 | ENST00000635359.1 | 333 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CSNK1G1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CSNK1G1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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