CRISPR-U™ Gene Knockout Cell Line Strategy

CSNK1G1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CSNK1G1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CSNK1G1
Gene id 53944
Organism Homo sapiens
Official full symbol casein kinase 1 gamma 1
Gene type protein-coding
Also known as CK1gamma1
Summary This gene encodes a member of the casein kinase I gene family. This family is comprised of serine/threonine kinases that phosphorylate acidic proteins such as caseins. The encoded kinase plays a role in cell cycle checkpoint arrest in response to stalled replication forks by phosphorylating Claspin. A mutation in this gene may be associated with non-syndromic early-onset epilepsy (NSEOE).
Genomic regions Chromosome 15
Strategy Summary
This gene has 14 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CSNK1G1-201 ENST00000303052.13 8085 422aa Protein coding CCDS10192 Q9HCP0-1 NM_022048.5 TSL:1, GENCODE basic, MANE Select v0.92,
CSNK1G1-205 ENST00000607537.6 2083 475aa Protein coding CCDS86465 U3KQB3 - TSL:1, GENCODE basic,
CSNK1G1-202 ENST00000561349.6 1756 438aa Protein coding CCDS86464 Q8IXA3 - TSL:1, GENCODE basic,
CSNK1G1-211 ENST00000635142.1 5548 430aa Protein coding - A0A0U1RQT7 - TSL:5, GENCODE basic,
CSNK1G1-209 ENST00000634811.1 2592 459aa Protein coding - A0A0U1RQY7 - TSL:5, GENCODE basic, APPRIS P1,
CSNK1G1-207 ENST00000634654.1 2485 459aa Protein coding - A0A0U1RQY7 - TSL:5, GENCODE basic, APPRIS P1,
CSNK1G1-204 ENST00000606793.3 1658 422aa Protein coding - U3KQA5 - TSL:5, GENCODE basic,
CSNK1G1-213 ENST00000635270.1 830 183aa Protein coding - A0A0U1RR14 - CDS 3' incomplete, TSL:5,
CSNK1G1-217 ENST00000635514.1 790 138aa Protein coding - A0A0U1RQK2 - CDS 3' incomplete, TSL:5,
CSNK1G1-206 ENST00000634302.1 712 204aa Protein coding - A0A0U1RQK6 - CDS 5' incomplete, TSL:5,
CSNK1G1-210 ENST00000634909.1 615 173aa Protein coding - A0A0U1RQG9 - CDS 5' incomplete, TSL:5,
CSNK1G1-208 ENST00000634722.1 601 158aa Protein coding - A0A0U1RR31 - CDS 5' incomplete, TSL:2,
CSNK1G1-216 ENST00000635414.1 600 67aa Protein coding - A0A0U1RQN5 - CDS 5' incomplete, TSL:3,
CSNK1G1-212 ENST00000635230.1 463 36aa Protein coding - A0A0U1RQU4 - CDS 5' incomplete, TSL:3,
CSNK1G1-203 ENST00000606225.1 1950 393aa Nonsense mediated decay - Q9HCP0-2 - TSL:1,
CSNK1G1-218 ENST00000635529.1 814 77aa Nonsense mediated decay - A0A0U1RRH5 - TSL:5,
CSNK1G1-214 ENST00000635324.1 799 114aa Nonsense mediated decay - A0A0U1RQH9 - TSL:5,
CSNK1G1-215 ENST00000635359.1 333 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CSNK1G1
This KO Strategy loading
Red Cotton™ Notes Gene CSNK1G1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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