CRISPR-U™ Gene Knockout Cell Line Strategy

PMAIP1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PMAIP1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PMAIP1
Gene id 5366
Organism Homo sapiens
Official full symbol phorbol-12-myristate-13-acetate-induced protein 1
Gene type protein-coding
Also known as APR, NOXA
Genomic regions Chromosome 18
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PMAIP1-202 ENST00000316660.7 1899 54aa Protein coding CCDS11975 Q13794-1 NM_021127.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PMAIP1-201 ENST00000269518.9 1262 136aa Protein coding - Q13794-2 - TSL:1, GENCODE basic,
PMAIP1-203 ENST00000590596.1 804 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PMAIP1
This KO Strategy loading
Red Cotton™ Notes Gene PMAIP1 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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