CRISPR-U™ Gene Knockout Cell Line Strategy

PLSCR1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PLSCR1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PLSCR1
Gene id 5359
Organism Homo sapiens
Official full symbol phospholipid scramblase 1
Gene type protein-coding
Also known as MMTRA1B
Genomic regions Chromosome 3
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PLSCR1-201 ENST00000342435.9 1976 318aa Protein coding CCDS3135 O15162-1 NM_021105.3 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
PLSCR1-214 ENST00000487389.5 1443 311aa Protein coding CCDS87153 C9J7K9 - TSL:5, GENCODE basic, APPRIS ALT2,
PLSCR1-202 ENST00000448787.6 996 237aa Protein coding CCDS87152 O15162-2 - TSL:2, GENCODE basic,
PLSCR1-208 ENST00000472349.1 1198 249aa Protein coding - C9JE06 - CDS 3' incomplete, TSL:5,
PLSCR1-203 ENST00000462666.5 862 248aa Protein coding - C9J9P4 - CDS 3' incomplete, TSL:5,
PLSCR1-213 ENST00000486631.5 701 171aa Protein coding - C9J0H3 - CDS 3' incomplete, TSL:5,
PLSCR1-211 ENST00000483300.5 514 171aa Protein coding - H7C5I5 - CDS 5' and 3' incomplete, TSL:5,
PLSCR1-218 ENST00000493432.5 1384 41aa Nonsense mediated decay - F2Z3F2 - TSL:1,
PLSCR1-216 ENST00000489775.5 984 41aa Nonsense mediated decay - F2Z3F2 - TSL:5,
PLSCR1-205 ENST00000468985.5 983 41aa Nonsense mediated decay - F2Z3F2 - TSL:5,
PLSCR1-210 ENST00000478267.5 870 41aa Nonsense mediated decay - F2Z3F2 - TSL:2,
PLSCR1-204 ENST00000463777.5 701 41aa Nonsense mediated decay - F2Z3F2 - TSL:3,
PLSCR1-215 ENST00000488253.5 581 41aa Nonsense mediated decay - F2Z3F2 - TSL:3,
PLSCR1-212 ENST00000484560.1 629 No protein Processed transcript - - - TSL:5,
PLSCR1-209 ENST00000477974.1 415 No protein Processed transcript - - - TSL:3,
PLSCR1-207 ENST00000470496.1 340 No protein Processed transcript - - - TSL:5,
PLSCR1-206 ENST00000469266.1 1257 No protein Retained intron - - - TSL:1,
PLSCR1-219 ENST00000494568.5 817 No protein Retained intron - - - TSL:2,
PLSCR1-217 ENST00000490745.5 480 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PLSCR1
This KO Strategy loading
Red Cotton™ Notes Gene PLSCR1 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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