CRISPR-U™ Gene Knockout Cell Line
Strategy
PLS3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PLS3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PLS3 |
| Gene id | 5358 |
| Organism | Homo sapiens |
| Official full symbol | plastin 3 |
| Gene type | protein-coding |
| Also known as | BMND18, T-plastin |
| Summary | Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PLS3-202 | ENST00000355899.8 | 3288 | 630aa | Protein coding | CCDS14568 | P13797-1 | NM_005032.7 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PLS3-209 | ENST00000539310.5 | 3037 | 630aa | Protein coding | CCDS14568 | P13797-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PLS3-201 | ENST00000289290.7 | 2277 | 617aa | Protein coding | CCDS78499 | P13797-2 | - | TSL:2, GENCODE basic, |
| PLS3-203 | ENST00000420625.6 | 3150 | 617aa | Protein coding | - | A0A0A0MSQ0 | - | TSL:5, GENCODE basic, |
| PLS3-208 | ENST00000497870.1 | 568 | 190aa | Protein coding | - | H7C4N2 | - | CDS 5' and 3' incomplete, TSL:4, |
| PLS3-205 | ENST00000473026.6 | 500 | 164aa | Protein coding | - | U3KQI3 | - | CDS 3' incomplete, TSL:3, |
| PLS3-210 | ENST00000626746.2 | 216 | 42aa | Protein coding | - | F2Z2Z9 | - | TSL:4, GENCODE basic, |
| PLS3-206 | ENST00000481823.5 | 2858 | 42aa | Nonsense mediated decay | - | F2Z2Z9 | - | TSL:2, |
| PLS3-207 | ENST00000489283.5 | 926 | 42aa | Nonsense mediated decay | - | F2Z2Z9 | - | TSL:1, |
| PLS3-204 | ENST00000466150.1 | 561 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PLS3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PLS3 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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