CRISPR-U™ Gene Knockout Cell Line Strategy

PLS3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PLS3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PLS3
Gene id 5358
Organism Homo sapiens
Official full symbol plastin 3
Gene type protein-coding
Also known as BMND18, T-plastin
Summary Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.
Genomic regions Chromosome X
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PLS3-202 ENST00000355899.8 3288 630aa Protein coding CCDS14568 P13797-1 NM_005032.7 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PLS3-209 ENST00000539310.5 3037 630aa Protein coding CCDS14568 P13797-1 - TSL:1, GENCODE basic, APPRIS P1,
PLS3-201 ENST00000289290.7 2277 617aa Protein coding CCDS78499 P13797-2 - TSL:2, GENCODE basic,
PLS3-203 ENST00000420625.6 3150 617aa Protein coding - A0A0A0MSQ0 - TSL:5, GENCODE basic,
PLS3-208 ENST00000497870.1 568 190aa Protein coding - H7C4N2 - CDS 5' and 3' incomplete, TSL:4,
PLS3-205 ENST00000473026.6 500 164aa Protein coding - U3KQI3 - CDS 3' incomplete, TSL:3,
PLS3-210 ENST00000626746.2 216 42aa Protein coding - F2Z2Z9 - TSL:4, GENCODE basic,
PLS3-206 ENST00000481823.5 2858 42aa Nonsense mediated decay - F2Z2Z9 - TSL:2,
PLS3-207 ENST00000489283.5 926 42aa Nonsense mediated decay - F2Z2Z9 - TSL:1,
PLS3-204 ENST00000466150.1 561 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PLS3
This KO Strategy loading
Red Cotton™ Notes Gene PLS3 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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