CRISPR-U™ Gene Knockout Cell Line Strategy

PLCG1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PLCG1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PLCG1
Gene id 5335
Organism Homo sapiens
Official full symbol phospholipase C gamma 1
Gene type protein-coding
Also known as NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1
Summary The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 20
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PLCG1-202 ENST00000373271.5 7395 1290aa Protein coding CCDS13314 P19174-1 - TSL:1, GENCODE basic, APPRIS ALT1,
PLCG1-201 ENST00000244007.7 5285 1291aa Protein coding CCDS13313 P19174-2 - TSL:5, GENCODE basic, APPRIS P3,
PLCG1-219 ENST00000612731.2 544 132aa Protein coding - A0A0D9SEK2 - CDS 5' incomplete, TSL:3,
PLCG1-214 ENST00000607954.1 1005 108aa Nonsense mediated decay - V9GY71 - CDS 5' incomplete, TSL:5,
PLCG1-217 ENST00000609257.5 956 133aa Nonsense mediated decay - V9GYH5 - CDS 5' incomplete, TSL:5,
PLCG1-213 ENST00000599785.5 880 50aa Nonsense mediated decay - M0QX77 - CDS 5' incomplete, TSL:5,
PLCG1-216 ENST00000608885.5 865 219aa Nonsense mediated decay - V9GY63 - CDS 5' incomplete, TSL:5,
PLCG1-215 ENST00000608689.5 969 No protein Processed transcript - - - TSL:5,
PLCG1-222 ENST00000630423.1 635 No protein Processed transcript - - - TSL:5,
PLCG1-210 ENST00000483646.2 591 No protein Processed transcript - - - TSL:3,
PLCG1-220 ENST00000617873.4 583 No protein Processed transcript - - - TSL:3,
PLCG1-218 ENST00000609821.5 570 No protein Processed transcript - - - TSL:5,
PLCG1-203 ENST00000423733.3 498 No protein Processed transcript - - - TSL:3,
PLCG1-221 ENST00000619272.2 330 No protein Processed transcript - - - TSL:3,
PLCG1-209 ENST00000483175.5 1084 No protein Retained intron - - - TSL:2,
PLCG1-208 ENST00000477870.5 1051 No protein Retained intron - - - TSL:2,
PLCG1-204 ENST00000461641.5 936 No protein Retained intron - - - TSL:5,
PLCG1-212 ENST00000492148.5 597 No protein Retained intron - - - TSL:2,
PLCG1-205 ENST00000465571.2 543 No protein Retained intron - - - TSL:2,
PLCG1-211 ENST00000490253.1 527 No protein Retained intron - - - TSL:2,
PLCG1-206 ENST00000470528.1 510 No protein Retained intron - - - TSL:4,
PLCG1-207 ENST00000473632.1 412 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PLCG1
This KO Strategy loading
Red Cotton™ Notes Gene PLCG1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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