CRISPR-U™ Gene Knockout Cell Line
Strategy
PLCG1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PLCG1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PLCG1 |
| Gene id | 5335 |
| Organism | Homo sapiens |
| Official full symbol | phospholipase C gamma 1 |
| Gene type | protein-coding |
| Also known as | NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1 |
| Summary | The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 20 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PLCG1-202 | ENST00000373271.5 | 7395 | 1290aa | Protein coding | CCDS13314 | P19174-1 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| PLCG1-201 | ENST00000244007.7 | 5285 | 1291aa | Protein coding | CCDS13313 | P19174-2 | - | TSL:5, GENCODE basic, APPRIS P3, |
| PLCG1-219 | ENST00000612731.2 | 544 | 132aa | Protein coding | - | A0A0D9SEK2 | - | CDS 5' incomplete, TSL:3, |
| PLCG1-214 | ENST00000607954.1 | 1005 | 108aa | Nonsense mediated decay | - | V9GY71 | - | CDS 5' incomplete, TSL:5, |
| PLCG1-217 | ENST00000609257.5 | 956 | 133aa | Nonsense mediated decay | - | V9GYH5 | - | CDS 5' incomplete, TSL:5, |
| PLCG1-213 | ENST00000599785.5 | 880 | 50aa | Nonsense mediated decay | - | M0QX77 | - | CDS 5' incomplete, TSL:5, |
| PLCG1-216 | ENST00000608885.5 | 865 | 219aa | Nonsense mediated decay | - | V9GY63 | - | CDS 5' incomplete, TSL:5, |
| PLCG1-215 | ENST00000608689.5 | 969 | No protein | Processed transcript | - | - | - | TSL:5, |
| PLCG1-222 | ENST00000630423.1 | 635 | No protein | Processed transcript | - | - | - | TSL:5, |
| PLCG1-210 | ENST00000483646.2 | 591 | No protein | Processed transcript | - | - | - | TSL:3, |
| PLCG1-220 | ENST00000617873.4 | 583 | No protein | Processed transcript | - | - | - | TSL:3, |
| PLCG1-218 | ENST00000609821.5 | 570 | No protein | Processed transcript | - | - | - | TSL:5, |
| PLCG1-203 | ENST00000423733.3 | 498 | No protein | Processed transcript | - | - | - | TSL:3, |
| PLCG1-221 | ENST00000619272.2 | 330 | No protein | Processed transcript | - | - | - | TSL:3, |
| PLCG1-209 | ENST00000483175.5 | 1084 | No protein | Retained intron | - | - | - | TSL:2, |
| PLCG1-208 | ENST00000477870.5 | 1051 | No protein | Retained intron | - | - | - | TSL:2, |
| PLCG1-204 | ENST00000461641.5 | 936 | No protein | Retained intron | - | - | - | TSL:5, |
| PLCG1-212 | ENST00000492148.5 | 597 | No protein | Retained intron | - | - | - | TSL:2, |
| PLCG1-205 | ENST00000465571.2 | 543 | No protein | Retained intron | - | - | - | TSL:2, |
| PLCG1-211 | ENST00000490253.1 | 527 | No protein | Retained intron | - | - | - | TSL:2, |
| PLCG1-206 | ENST00000470528.1 | 510 | No protein | Retained intron | - | - | - | TSL:4, |
| PLCG1-207 | ENST00000473632.1 | 412 | No protein | Retained intron | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PLCG1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PLCG1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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