CRISPR-U™ Gene Knockout Cell Line Strategy

PLCB3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PLCB3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PLCB3
Gene id 5331
Organism Homo sapiens
Official full symbol phospholipase C beta 3
Gene type protein-coding
Also known as SMDCD
Summary This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 11
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PLCB3-204 ENST00000540288.5 4469 1234aa Protein coding CCDS8064 Q01970-1 - TSL:1, GENCODE basic, APPRIS P1,
PLCB3-201 ENST00000279230.12 4192 1234aa Protein coding CCDS8064 Q01970-1 NM_000932.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PLCB3-202 ENST00000325234.5 3946 1167aa Protein coding CCDS53654 Q01970-2 - TSL:1, GENCODE basic,
PLCB3-203 ENST00000536243.1 471 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PLCB3
This KO Strategy loading
Red Cotton™ Notes Gene PLCB3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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