CRISPR-U™ Gene Knockout Cell Line
Strategy
PLCB3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PLCB3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PLCB3 |
| Gene id | 5331 |
| Organism | Homo sapiens |
| Official full symbol | phospholipase C beta 3 |
| Gene type | protein-coding |
| Also known as | SMDCD |
| Summary | This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PLCB3-204 | ENST00000540288.5 | 4469 | 1234aa | Protein coding | CCDS8064 | Q01970-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PLCB3-201 | ENST00000279230.12 | 4192 | 1234aa | Protein coding | CCDS8064 | Q01970-1 | NM_000932.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PLCB3-202 | ENST00000325234.5 | 3946 | 1167aa | Protein coding | CCDS53654 | Q01970-2 | - | TSL:1, GENCODE basic, |
| PLCB3-203 | ENST00000536243.1 | 471 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PLCB3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PLCB3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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