CRISPR-U™ Gene Knockout Cell Line Strategy

PIP4K2A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PIP4K2A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PIP4K2A
Gene id 5305
Organism Homo sapiens
Official full symbol phosphatidylinositol-5-phosphate 4-kinase type 2 alpha
Gene type protein-coding
Also known as PI5P4KA, PIP5K2A, PIP5KII-alpha, PIP5KIIA, PIPK
Summary Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family.
Genomic regions Chromosome 10
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PIP4K2A-202 ENST00000376573.9 3820 406aa Protein coding CCDS7141 P48426-1 NM_005028.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PIP4K2A-206 ENST00000545335.5 1436 347aa Protein coding CCDS81443 P48426-2 - TSL:2, GENCODE basic,
PIP4K2A-201 ENST00000323883.11 1319 266aa Protein coding - H7BXS3 - TSL:2, GENCODE basic,
PIP4K2A-207 ENST00000604912.1 443 142aa Protein coding - S4R320 - CDS 3' incomplete, TSL:2,
PIP4K2A-203 ENST00000422321.5 768 No protein Processed transcript - - - TSL:3,
PIP4K2A-205 ENST00000474335.1 559 No protein Processed transcript - - - TSL:2,
PIP4K2A-208 ENST00000605011.1 558 No protein Processed transcript - - - TSL:4,
PIP4K2A-204 ENST00000432610.1 304 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PIP4K2A
This KO Strategy loading
Red Cotton™ Notes Gene PIP4K2A had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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