CRISPR-U™ Gene Knockout Cell Line Strategy

PIK3R2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PIK3R2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PIK3R2
Gene id 5296
Organism Homo sapiens
Official full symbol phosphoinositide-3-kinase regulatory subunit 2
Gene type protein-coding
Also known as MPPH, MPPH1, P85B, p85, p85-BETA
Summary Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Three transcript variants, one protein coding and the other two non-protein coding, have been found for this gene.
Genomic regions Chromosome 19
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PIK3R2-201 ENST00000222254.13 3980 728aa Protein coding CCDS12371 O00459 NM_005027.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PIK3R2-207 ENST00000617130.5 3796 267aa Nonsense mediated decay - O00459 - TSL:1,
PIK3R2-208 ENST00000617642.2 3639 223aa Nonsense mediated decay - E9PFP1 - TSL:5,
PIK3R2-202 ENST00000426902.5 3273 481aa Nonsense mediated decay - E9PFP1 - TSL:2,
PIK3R2-209 ENST00000672815.1 741 48aa Nonsense mediated decay - A0A5F9ZHY3 - CDS 5' incomplete,
PIK3R2-204 ENST00000464016.3 588 124aa Nonsense mediated decay - V9GYJ4 - CDS 5' incomplete, TSL:3,
PIK3R2-211 ENST00000675271.1 1231 No protein Retained intron - - - -
PIK3R2-210 ENST00000674682.1 879 No protein Retained intron - - - -
PIK3R2-203 ENST00000459743.2 465 No protein Retained intron - - - TSL:2,
PIK3R2-205 ENST00000474310.1 425 No protein Retained intron - - - TSL:3,
PIK3R2-206 ENST00000600533.2 425 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PIK3R2
This KO Strategy loading
Red Cotton™ Notes Gene PIK3R2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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