CRISPR-U™ Gene Knockout Cell Line
Strategy
PIK3R2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PIK3R2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PIK3R2 |
| Gene id | 5296 |
| Organism | Homo sapiens |
| Official full symbol | phosphoinositide-3-kinase regulatory subunit 2 |
| Gene type | protein-coding |
| Also known as | MPPH, MPPH1, P85B, p85, p85-BETA |
| Summary | Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Three transcript variants, one protein coding and the other two non-protein coding, have been found for this gene. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PIK3R2-201 | ENST00000222254.13 | 3980 | 728aa | Protein coding | CCDS12371 | O00459 | NM_005027.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PIK3R2-207 | ENST00000617130.5 | 3796 | 267aa | Nonsense mediated decay | - | O00459 | - | TSL:1, |
| PIK3R2-208 | ENST00000617642.2 | 3639 | 223aa | Nonsense mediated decay | - | E9PFP1 | - | TSL:5, |
| PIK3R2-202 | ENST00000426902.5 | 3273 | 481aa | Nonsense mediated decay | - | E9PFP1 | - | TSL:2, |
| PIK3R2-209 | ENST00000672815.1 | 741 | 48aa | Nonsense mediated decay | - | A0A5F9ZHY3 | - | CDS 5' incomplete, |
| PIK3R2-204 | ENST00000464016.3 | 588 | 124aa | Nonsense mediated decay | - | V9GYJ4 | - | CDS 5' incomplete, TSL:3, |
| PIK3R2-211 | ENST00000675271.1 | 1231 | No protein | Retained intron | - | - | - | - |
| PIK3R2-210 | ENST00000674682.1 | 879 | No protein | Retained intron | - | - | - | - |
| PIK3R2-203 | ENST00000459743.2 | 465 | No protein | Retained intron | - | - | - | TSL:2, |
| PIK3R2-205 | ENST00000474310.1 | 425 | No protein | Retained intron | - | - | - | TSL:3, |
| PIK3R2-206 | ENST00000600533.2 | 425 | No protein | Retained intron | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PIK3R2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PIK3R2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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