CRISPR-U™ Gene Knockout Cell Line
Strategy
PIK3R1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PIK3R1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PIK3R1 |
| Gene id | 5295 |
| Organism | Homo sapiens |
| Official full symbol | phosphoinositide-3-kinase regulatory subunit 1 |
| Gene type | protein-coding |
| Also known as | AGM7, GRB1, IMD36, p85, p85-ALPHA |
| Summary | Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PIK3R1-211 | ENST00000521381.6 | 6975 | 724aa | Protein coding | CCDS3993 | P27986-1 | NM_181523.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PIK3R1-213 | ENST00000521657.5 | 3891 | 724aa | Protein coding | CCDS3993 | P27986-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| PIK3R1-201 | ENST00000320694.12 | 2625 | 424aa | Protein coding | CCDS3994 | P27986-3 | - | TSL:1, GENCODE basic, |
| PIK3R1-216 | ENST00000523872.1 | 2473 | 361aa | Protein coding | CCDS56374 | P27986-5 | - | TSL:2, GENCODE basic, |
| PIK3R1-202 | ENST00000336483.9 | 2439 | 454aa | Protein coding | CCDS3995 | P27986-2 | - | TSL:1, GENCODE basic, |
| PIK3R1-214 | ENST00000522084.5 | 705 | 102aa | Protein coding | - | E5RHI0 | - | CDS 3' incomplete, TSL:3, |
| PIK3R1-208 | ENST00000519025.5 | 567 | 189aa | Protein coding | - | H0YBC2 | - | CDS 5' and 3' incomplete, TSL:4, |
| PIK3R1-215 | ENST00000523807.5 | 552 | 117aa | Protein coding | - | E5RGI8 | - | CDS 3' incomplete, TSL:4, |
| PIK3R1-212 | ENST00000521409.5 | 540 | 137aa | Protein coding | - | E5RJY0 | - | CDS 3' incomplete, TSL:4, |
| PIK3R1-210 | ENST00000520675.1 | 395 | 131aa | Protein coding | - | H0YB27 | - | CDS 5' and 3' incomplete, TSL:5, |
| PIK3R1-205 | ENST00000517698.5 | 945 | 38aa | Nonsense mediated decay | - | E5RK66 | - | TSL:3, |
| PIK3R1-204 | ENST00000517643.1 | 586 | No protein | Processed transcript | - | - | - | TSL:3, |
| PIK3R1-207 | ENST00000518813.5 | 3722 | No protein | Retained intron | - | - | - | TSL:2, |
| PIK3R1-209 | ENST00000520550.1 | 1468 | No protein | Retained intron | - | - | - | TSL:2, |
| PIK3R1-203 | ENST00000517412.1 | 865 | No protein | Retained intron | - | - | - | TSL:1, |
| PIK3R1-206 | ENST00000518292.1 | 558 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PIK3R1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PIK3R1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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