CRISPR-U™ Gene Knockout Cell Line Strategy

PIK3R1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PIK3R1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PIK3R1
Gene id 5295
Organism Homo sapiens
Official full symbol phosphoinositide-3-kinase regulatory subunit 1
Gene type protein-coding
Also known as AGM7, GRB1, IMD36, p85, p85-ALPHA
Summary Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.
Genomic regions Chromosome 5
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PIK3R1-211 ENST00000521381.6 6975 724aa Protein coding CCDS3993 P27986-1 NM_181523.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PIK3R1-213 ENST00000521657.5 3891 724aa Protein coding CCDS3993 P27986-1 - TSL:5, GENCODE basic, APPRIS P1,
PIK3R1-201 ENST00000320694.12 2625 424aa Protein coding CCDS3994 P27986-3 - TSL:1, GENCODE basic,
PIK3R1-216 ENST00000523872.1 2473 361aa Protein coding CCDS56374 P27986-5 - TSL:2, GENCODE basic,
PIK3R1-202 ENST00000336483.9 2439 454aa Protein coding CCDS3995 P27986-2 - TSL:1, GENCODE basic,
PIK3R1-214 ENST00000522084.5 705 102aa Protein coding - E5RHI0 - CDS 3' incomplete, TSL:3,
PIK3R1-208 ENST00000519025.5 567 189aa Protein coding - H0YBC2 - CDS 5' and 3' incomplete, TSL:4,
PIK3R1-215 ENST00000523807.5 552 117aa Protein coding - E5RGI8 - CDS 3' incomplete, TSL:4,
PIK3R1-212 ENST00000521409.5 540 137aa Protein coding - E5RJY0 - CDS 3' incomplete, TSL:4,
PIK3R1-210 ENST00000520675.1 395 131aa Protein coding - H0YB27 - CDS 5' and 3' incomplete, TSL:5,
PIK3R1-205 ENST00000517698.5 945 38aa Nonsense mediated decay - E5RK66 - TSL:3,
PIK3R1-204 ENST00000517643.1 586 No protein Processed transcript - - - TSL:3,
PIK3R1-207 ENST00000518813.5 3722 No protein Retained intron - - - TSL:2,
PIK3R1-209 ENST00000520550.1 1468 No protein Retained intron - - - TSL:2,
PIK3R1-203 ENST00000517412.1 865 No protein Retained intron - - - TSL:1,
PIK3R1-206 ENST00000518292.1 558 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PIK3R1
This KO Strategy loading
Red Cotton™ Notes Gene PIK3R1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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