CRISPR-U™ Gene Knockout Cell Line Strategy

PHKA1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PHKA1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PHKA1
Gene id 5255
Organism Homo sapiens
Official full symbol phosphorylase kinase regulatory subunit alpha 1
Gene type protein-coding
Also known as PHKA
Summary Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.
Genomic regions Chromosome X
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PHKA1-203 ENST00000373542.9 6286 1223aa Protein coding CCDS14421 P46020-1 NM_002637.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
PHKA1-201 ENST00000339490.7 6121 1210aa Protein coding CCDS48137 P46020-2 - TSL:1, GENCODE basic, APPRIS ALT2,
PHKA1-205 ENST00000541944.5 5944 1151aa Protein coding CCDS55453 P46020-3 - TSL:1, GENCODE basic, APPRIS ALT2,
PHKA1-204 ENST00000373545.7 6173 1181aa Protein coding - A6NIT2 - TSL:5, GENCODE basic, APPRIS ALT2,
PHKA1-202 ENST00000373539.3 4476 1240aa Protein coding - A6NMN0 - TSL:5, GENCODE basic, APPRIS ALT2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PHKA1
This KO Strategy loading
Red Cotton™ Notes Gene PHKA1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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