CRISPR-U™ Gene Knockout Cell Line Strategy

PGR Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PGR Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PGR
Gene id 5241
Organism Homo sapiens
Official full symbol progesterone receptor
Gene type protein-coding
Also known as NR3C3, PR
Summary This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce several transcript variants, both protein coding and non-protein coding. Two of the isoforms (A and B) are identical except for an additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap.
Genomic regions Chromosome 11
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PGR-202 ENST00000325455.10 13037 933aa Protein coding CCDS8310 P06401-1 NM_000926.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PGR-207 ENST00000534013.5 1901 339aa Protein coding CCDS59229 P06401-3 - TSL:2, GENCODE basic,
PGR-210 ENST00000619228.2 3038 764aa Protein coding - Q8NG44 - TSL:5, GENCODE basic,
PGR-209 ENST00000617858.4 2816 690aa Protein coding - A0A087WXR4 - TSL:5, GENCODE basic,
PGR-201 ENST00000263463.9 2496 831aa Protein coding - P06401-5 - TSL:1, GENCODE basic,
PGR-211 ENST00000632634.1 551 170aa Protein coding - A0A0J9YVP1 - CDS 3' incomplete, TSL:3,
PGR-208 ENST00000534780.5 2750 803aa Nonsense mediated decay - Q8NG42 - TSL:1,
PGR-204 ENST00000528960.5 2633 764aa Nonsense mediated decay - Q8NG44 - TSL:1,
PGR-203 ENST00000526300.5 2365 695aa Nonsense mediated decay - Q8NG45 - TSL:1,
PGR-206 ENST00000533207.5 2224 No protein Retained intron - - - TSL:1,
PGR-205 ENST00000530764.1 620 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PGR
This KO Strategy loading
Red Cotton™ Notes Gene PGR had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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