CRISPR-U™ Gene Knockout Cell Line Strategy

PGM1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PGM1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PGM1
Gene id 5236
Organism Homo sapiens
Official full symbol phosphoglucomutase 1
Gene type protein-coding
Also known as CDG1T, GSD14
Summary The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PGM1-201 ENST00000371083.4 2657 580aa Protein coding CCDS53323 P36871-2 - TSL:2, GENCODE basic,
PGM1-205 ENST00000540265.5 2345 365aa Protein coding CCDS53324 P36871-3 - TSL:2, GENCODE basic,
PGM1-202 ENST00000371084.8 2337 562aa Protein coding CCDS625 P36871-1 NM_002633.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PGM1-206 ENST00000650546.1 2423 584aa Protein coding - A0A3B3ITK7 - GENCODE basic,
PGM1-204 ENST00000483707.1 612 No protein Processed transcript - - - TSL:2,
PGM1-203 ENST00000473117.2 373 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PGM1
This KO Strategy loading
Red Cotton™ Notes Gene PGM1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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