CRISPR-U™ Gene Knockout Cell Line
Strategy
PGM1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PGM1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PGM1 |
| Gene id | 5236 |
| Organism | Homo sapiens |
| Official full symbol | phosphoglucomutase 1 |
| Gene type | protein-coding |
| Also known as | CDG1T, GSD14 |
| Summary | The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PGM1-201 | ENST00000371083.4 | 2657 | 580aa | Protein coding | CCDS53323 | P36871-2 | - | TSL:2, GENCODE basic, |
| PGM1-205 | ENST00000540265.5 | 2345 | 365aa | Protein coding | CCDS53324 | P36871-3 | - | TSL:2, GENCODE basic, |
| PGM1-202 | ENST00000371084.8 | 2337 | 562aa | Protein coding | CCDS625 | P36871-1 | NM_002633.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PGM1-206 | ENST00000650546.1 | 2423 | 584aa | Protein coding | - | A0A3B3ITK7 | - | GENCODE basic, |
| PGM1-204 | ENST00000483707.1 | 612 | No protein | Processed transcript | - | - | - | TSL:2, |
| PGM1-203 | ENST00000473117.2 | 373 | No protein | Processed transcript | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PGM1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PGM1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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