CRISPR-U™ Gene Knockout Cell Line Strategy

PFKP Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PFKP Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PFKP
Gene id 5214
Organism Homo sapiens
Official full symbol phosphofructokinase, platelet
Gene type protein-coding
Also known as ATP-PFK, PFK-C, PFK-P, PFKF
Summary This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 10
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PFKP-203 ENST00000381125.9 2626 784aa Protein coding CCDS7059 Q01813-1 NM_002627.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PFKP-206 ENST00000415005.6 3265 568aa Protein coding - B1APP6 - TSL:2, GENCODE basic,
PFKP-213 ENST00000676796.1 2759 746aa Protein coding - - - -
PFKP-202 ENST00000381075.7 2741 746aa Protein coding - Q01813-2 - TSL:2, GENCODE basic,
PFKP-204 ENST00000407806.2 705 210aa Protein coding - Q5VSR5 - CDS 3' incomplete, TSL:5,
PFKP-205 ENST00000413079.1 496 166aa Protein coding - H0Y3Y3 - CDS 5' and 3' incomplete, TSL:3,
PFKP-208 ENST00000433193.2 456 103aa Protein coding - H0Y757 - CDS 5' incomplete, TSL:3,
PFKP-212 ENST00000607886.5 432 113aa Protein coding - V9GYV7 - CDS 3' incomplete, TSL:5,
PFKP-210 ENST00000468050.1 425 118aa Protein coding - V9GY25 - CDS 3' incomplete, TSL:3,
PFKP-215 ENST00000678089.1 2769 52aa Nonsense mediated decay - - - -
PFKP-216 ENST00000678206.1 2685 122aa Nonsense mediated decay - - - -
PFKP-214 ENST00000677351.1 2061 No protein Processed transcript - - - -
PFKP-207 ENST00000421751.1 658 No protein Processed transcript - - - TSL:4,
PFKP-209 ENST00000460445.1 552 No protein Processed transcript - - - TSL:5,
PFKP-211 ENST00000495715.1 143 No protein Processed transcript - - - TSL:3,
PFKP-201 ENST00000381072.5 1698 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PFKP
This KO Strategy loading
Red Cotton™ Notes Gene PFKP had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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