CRISPR-U™ Gene Knockout Cell Line
Strategy
PFKP Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PFKP Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PFKP |
| Gene id | 5214 |
| Organism | Homo sapiens |
| Official full symbol | phosphofructokinase, platelet |
| Gene type | protein-coding |
| Also known as | ATP-PFK, PFK-C, PFK-P, PFKF |
| Summary | This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PFKP-203 | ENST00000381125.9 | 2626 | 784aa | Protein coding | CCDS7059 | Q01813-1 | NM_002627.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PFKP-206 | ENST00000415005.6 | 3265 | 568aa | Protein coding | - | B1APP6 | - | TSL:2, GENCODE basic, |
| PFKP-213 | ENST00000676796.1 | 2759 | 746aa | Protein coding | - | - | - | - |
| PFKP-202 | ENST00000381075.7 | 2741 | 746aa | Protein coding | - | Q01813-2 | - | TSL:2, GENCODE basic, |
| PFKP-204 | ENST00000407806.2 | 705 | 210aa | Protein coding | - | Q5VSR5 | - | CDS 3' incomplete, TSL:5, |
| PFKP-205 | ENST00000413079.1 | 496 | 166aa | Protein coding | - | H0Y3Y3 | - | CDS 5' and 3' incomplete, TSL:3, |
| PFKP-208 | ENST00000433193.2 | 456 | 103aa | Protein coding | - | H0Y757 | - | CDS 5' incomplete, TSL:3, |
| PFKP-212 | ENST00000607886.5 | 432 | 113aa | Protein coding | - | V9GYV7 | - | CDS 3' incomplete, TSL:5, |
| PFKP-210 | ENST00000468050.1 | 425 | 118aa | Protein coding | - | V9GY25 | - | CDS 3' incomplete, TSL:3, |
| PFKP-215 | ENST00000678089.1 | 2769 | 52aa | Nonsense mediated decay | - | - | - | - |
| PFKP-216 | ENST00000678206.1 | 2685 | 122aa | Nonsense mediated decay | - | - | - | - |
| PFKP-214 | ENST00000677351.1 | 2061 | No protein | Processed transcript | - | - | - | - |
| PFKP-207 | ENST00000421751.1 | 658 | No protein | Processed transcript | - | - | - | TSL:4, |
| PFKP-209 | ENST00000460445.1 | 552 | No protein | Processed transcript | - | - | - | TSL:5, |
| PFKP-211 | ENST00000495715.1 | 143 | No protein | Processed transcript | - | - | - | TSL:3, |
| PFKP-201 | ENST00000381072.5 | 1698 | No protein | Retained intron | - | - | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PFKP |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PFKP had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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