CRISPR-U™ Gene Knockout Cell Line
Strategy
PER1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PER1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PER1 |
| Gene id | 5187 |
| Organism | Homo sapiens |
| Official full symbol | period circadian regulator 1 |
| Gene type | protein-coding |
| Also known as | PER, RIGUI, hPER |
| Summary | This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers. Alternative splicing has been observed in this gene; however, these variants have not been fully described. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PER1-201 | ENST00000317276.9 | 4676 | 1290aa | Protein coding | CCDS11131 | O15534-1 | NM_002616.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PER1-211 | ENST00000581082.5 | 4521 | 1267aa | Protein coding | - | J3KRL7 | - | TSL:5, GENCODE basic, |
| PER1-202 | ENST00000354903.9 | 3115 | 859aa | Protein coding | - | O15534-4 | - | TSL:2, GENCODE basic, |
| PER1-203 | ENST00000577253.5 | 589 | 117aa | Protein coding | - | J3KSL6 | - | CDS 3' incomplete, TSL:4, |
| PER1-217 | ENST00000584202.1 | 576 | 112aa | Protein coding | - | J3QLQ5 | - | CDS 3' incomplete, TSL:5, |
| PER1-213 | ENST00000581703.1 | 528 | 175aa | Protein coding | - | J3QL55 | - | CDS 3' incomplete, TSL:4, |
| PER1-215 | ENST00000583559.1 | 523 | 168aa | Protein coding | - | J3QL46 | - | CDS 5' incomplete, TSL:2, |
| PER1-214 | ENST00000582719.5 | 3677 | 824aa | Nonsense mediated decay | - | J3KTM2 | - | TSL:5, |
| PER1-212 | ENST00000581395.5 | 3209 | 572aa | Nonsense mediated decay | - | J3QSH9 | - | TSL:2, |
| PER1-205 | ENST00000578089.1 | 830 | No protein | Processed transcript | - | - | - | TSL:3, |
| PER1-208 | ENST00000579065.1 | 1262 | No protein | Retained intron | - | - | - | TSL:5, |
| PER1-206 | ENST00000578223.1 | 717 | No protein | Retained intron | - | - | - | TSL:5, |
| PER1-218 | ENST00000585095.1 | 691 | No protein | Retained intron | - | - | - | TSL:3, |
| PER1-204 | ENST00000577424.1 | 626 | No protein | Retained intron | - | - | - | TSL:3, |
| PER1-210 | ENST00000579203.1 | 564 | No protein | Retained intron | - | - | - | TSL:4, |
| PER1-207 | ENST00000578950.1 | 540 | No protein | Retained intron | - | - | - | TSL:2, |
| PER1-216 | ENST00000583677.1 | 512 | No protein | Retained intron | - | - | - | TSL:1, |
| PER1-219 | ENST00000585284.1 | 461 | No protein | Retained intron | - | - | - | TSL:2, |
| PER1-209 | ENST00000579098.1 | 367 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PER1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PER1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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