CRISPR-U™ Gene Knockout Cell Line Strategy

PER1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PER1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PER1
Gene id 5187
Organism Homo sapiens
Official full symbol period circadian regulator 1
Gene type protein-coding
Also known as PER, RIGUI, hPER
Summary This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers. Alternative splicing has been observed in this gene; however, these variants have not been fully described.
Genomic regions Chromosome 17
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PER1-201 ENST00000317276.9 4676 1290aa Protein coding CCDS11131 O15534-1 NM_002616.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PER1-211 ENST00000581082.5 4521 1267aa Protein coding - J3KRL7 - TSL:5, GENCODE basic,
PER1-202 ENST00000354903.9 3115 859aa Protein coding - O15534-4 - TSL:2, GENCODE basic,
PER1-203 ENST00000577253.5 589 117aa Protein coding - J3KSL6 - CDS 3' incomplete, TSL:4,
PER1-217 ENST00000584202.1 576 112aa Protein coding - J3QLQ5 - CDS 3' incomplete, TSL:5,
PER1-213 ENST00000581703.1 528 175aa Protein coding - J3QL55 - CDS 3' incomplete, TSL:4,
PER1-215 ENST00000583559.1 523 168aa Protein coding - J3QL46 - CDS 5' incomplete, TSL:2,
PER1-214 ENST00000582719.5 3677 824aa Nonsense mediated decay - J3KTM2 - TSL:5,
PER1-212 ENST00000581395.5 3209 572aa Nonsense mediated decay - J3QSH9 - TSL:2,
PER1-205 ENST00000578089.1 830 No protein Processed transcript - - - TSL:3,
PER1-208 ENST00000579065.1 1262 No protein Retained intron - - - TSL:5,
PER1-206 ENST00000578223.1 717 No protein Retained intron - - - TSL:5,
PER1-218 ENST00000585095.1 691 No protein Retained intron - - - TSL:3,
PER1-204 ENST00000577424.1 626 No protein Retained intron - - - TSL:3,
PER1-210 ENST00000579203.1 564 No protein Retained intron - - - TSL:4,
PER1-207 ENST00000578950.1 540 No protein Retained intron - - - TSL:2,
PER1-216 ENST00000583677.1 512 No protein Retained intron - - - TSL:1,
PER1-219 ENST00000585284.1 461 No protein Retained intron - - - TSL:2,
PER1-209 ENST00000579098.1 367 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PER1
This KO Strategy loading
Red Cotton™ Notes Gene PER1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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