CRISPR-U™ Gene Knockout Cell Line Strategy

INPP5K Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human INPP5K Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol INPP5K
Gene id 51763
Organism Homo sapiens
Official full symbol inositol polyphosphate-5-phosphatase K
Gene type protein-coding
Also known as MDCCAID, PPS, SKIP
Summary This gene encodes a protein with 5-phosphatase activity toward polyphosphate inositol. The protein localizes to the cytosol in regions lacking actin stress fibers. It is thought that this protein may negatively regulate the actin cytoskeleton. Alternatively spliced transcript variants encoding different isoforms have been identified.
Genomic regions Chromosome 17
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
INPP5K-203 ENST00000406424.8 3194 372aa Protein coding CCDS11005 - - TSL:5, GENCODE basic,
INPP5K-201 ENST00000320345.10 2880 372aa Protein coding CCDS11005 - - TSL:5, GENCODE basic,
INPP5K-204 ENST00000421807.7 2706 448aa Protein coding CCDS11004 Q9BT40 NM_016532.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
INPP5K-206 ENST00000449479.5 949 229aa Protein coding - C9JZB0 - CDS 3' incomplete, TSL:2,
INPP5K-210 ENST00000477910.5 739 50aa Protein coding - K7EPG6 - CDS 3' incomplete, TSL:3,
INPP5K-214 ENST00000498390.5 622 23aa Protein coding - K7ENF7 - CDS 3' incomplete, TSL:4,
INPP5K-219 ENST00000575172.5 594 158aa Protein coding - I3L2L1 - CDS 3' incomplete, TSL:4,
INPP5K-217 ENST00000574561.1 591 154aa Protein coding - I3L4A5 - CDS 3' incomplete, TSL:4,
INPP5K-215 ENST00000571274.5 571 84aa Protein coding - I3L0Y0 - CDS 3' incomplete, TSL:4,
INPP5K-202 ENST00000350761.9 1505 63aa Nonsense mediated decay - J3KN07 - TSL:2,
INPP5K-216 ENST00000573790.5 635 99aa Nonsense mediated decay - I3NI31 - TSL:3,
INPP5K-220 ENST00000576646.7 629 38aa Nonsense mediated decay - K7EMS8 - CDS 5' incomplete, TSL:5,
INPP5K-218 ENST00000574955.1 566 51aa Nonsense mediated decay - I3L1R1 - TSL:4,
INPP5K-205 ENST00000445774.2 565 52aa Nonsense mediated decay - C9JQW8 - TSL:4,
INPP5K-209 ENST00000477115.1 770 No protein Retained intron - - - TSL:3,
INPP5K-208 ENST00000461552.1 581 No protein Retained intron - - - TSL:2,
INPP5K-211 ENST00000481867.1 558 No protein Retained intron - - - TSL:3,
INPP5K-207 ENST00000460733.5 554 No protein Retained intron - - - TSL:2,
INPP5K-212 ENST00000487039.1 482 No protein Retained intron - - - TSL:2,
INPP5K-213 ENST00000495339.1 406 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene INPP5K
This KO Strategy loading
Red Cotton™ Notes Gene INPP5K had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×