CRISPR-U™ Gene Knockout Cell Line Strategy

CDK12 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CDK12 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDK12
Gene id 51755
Organism Homo sapiens
Official full symbol cyclin dependent kinase 12
Gene type protein-coding
Also known as CRK7, CRKR, CRKRS
Genomic regions Chromosome 17
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDK12-202 ENST00000447079.6 8287 1490aa Protein coding CCDS11337 Q9NYV4-1 NM_016507.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
CDK12-201 ENST00000430627.6 5918 1481aa Protein coding CCDS45666 Q9NYV4-2 - TSL:1, GENCODE basic, APPRIS ALT2,
CDK12-209 ENST00000584632.5 4165 1201aa Protein coding - J3QSD7 - CDS 3' incomplete, TSL:5,
CDK12-206 ENST00000581593.1 559 186aa Protein coding - A0A590UJE9 - CDS 5' and 3' incomplete, TSL:3,
CDK12-205 ENST00000559663.1 871 159aa Nonsense mediated decay - H0YLT2 - CDS 5' incomplete, TSL:5,
CDK12-204 ENST00000559545.5 586 No protein Processed transcript - - - TSL:5,
CDK12-203 ENST00000558240.1 555 No protein Processed transcript - - - TSL:4,
CDK12-210 ENST00000585161.1 2036 No protein Retained intron - - - TSL:NA,
CDK12-208 ENST00000584336.1 1963 No protein Retained intron - - - TSL:NA,
CDK12-207 ENST00000581963.1 436 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDK12
This KO Strategy loading
Red Cotton™ Notes Gene CDK12 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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