CRISPR-U™ Gene Knockout Cell Line
Strategy
CDK12 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CDK12 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CDK12 |
| Gene id | 51755 |
| Organism | Homo sapiens |
| Official full symbol | cyclin dependent kinase 12 |
| Gene type | protein-coding |
| Also known as | CRK7, CRKR, CRKRS |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CDK12-202 | ENST00000447079.6 | 8287 | 1490aa | Protein coding | CCDS11337 | Q9NYV4-1 | NM_016507.4 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| CDK12-201 | ENST00000430627.6 | 5918 | 1481aa | Protein coding | CCDS45666 | Q9NYV4-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| CDK12-209 | ENST00000584632.5 | 4165 | 1201aa | Protein coding | - | J3QSD7 | - | CDS 3' incomplete, TSL:5, |
| CDK12-206 | ENST00000581593.1 | 559 | 186aa | Protein coding | - | A0A590UJE9 | - | CDS 5' and 3' incomplete, TSL:3, |
| CDK12-205 | ENST00000559663.1 | 871 | 159aa | Nonsense mediated decay | - | H0YLT2 | - | CDS 5' incomplete, TSL:5, |
| CDK12-204 | ENST00000559545.5 | 586 | No protein | Processed transcript | - | - | - | TSL:5, |
| CDK12-203 | ENST00000558240.1 | 555 | No protein | Processed transcript | - | - | - | TSL:4, |
| CDK12-210 | ENST00000585161.1 | 2036 | No protein | Retained intron | - | - | - | TSL:NA, |
| CDK12-208 | ENST00000584336.1 | 1963 | No protein | Retained intron | - | - | - | TSL:NA, |
| CDK12-207 | ENST00000581963.1 | 436 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CDK12 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CDK12 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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