CRISPR-U™ Gene Knockout Cell Line Strategy

NLK Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NLK Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NLK
Gene id 51701
Organism Homo sapiens
Official full symbol nemo like kinase
Gene type protein-coding
Genomic regions Chromosome 17
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NLK-201 ENST00000407008.8 3526 527aa Protein coding CCDS11224 A0A024QZ12 Q9UBE8 NM_016231.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NLK-203 ENST00000582037.2 1503 181aa Protein coding - J3QSE9 - TSL:2, GENCODE basic,
NLK-202 ENST00000496808.1 1702 475aa Nonsense mediated decay - H0YD75 - CDS 5' incomplete, TSL:2,
NLK-204 ENST00000583517.1 695 No protein Processed transcript - - - TSL:3,
NLK-205 ENST00000584188.1 451 No protein Processed transcript - - - TSL:3,
NLK-206 ENST00000584878.1 777 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NLK
This KO Strategy loading
Red Cotton™ Notes Gene NLK had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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