CRISPR-U™ Gene Knockout Cell Line
Strategy
NLK Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NLK Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NLK |
| Gene id | 51701 |
| Organism | Homo sapiens |
| Official full symbol | nemo like kinase |
| Gene type | protein-coding |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NLK-201 | ENST00000407008.8 | 3526 | 527aa | Protein coding | CCDS11224 | A0A024QZ12 Q9UBE8 | NM_016231.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NLK-203 | ENST00000582037.2 | 1503 | 181aa | Protein coding | - | J3QSE9 | - | TSL:2, GENCODE basic, |
| NLK-202 | ENST00000496808.1 | 1702 | 475aa | Nonsense mediated decay | - | H0YD75 | - | CDS 5' incomplete, TSL:2, |
| NLK-204 | ENST00000583517.1 | 695 | No protein | Processed transcript | - | - | - | TSL:3, |
| NLK-205 | ENST00000584188.1 | 451 | No protein | Processed transcript | - | - | - | TSL:3, |
| NLK-206 | ENST00000584878.1 | 777 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NLK |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NLK had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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