CRISPR-U™ Gene Knockout Cell Line
Strategy
SUFU Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SUFU Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SUFU |
| Gene id | 51684 |
| Organism | Homo sapiens |
| Official full symbol | SUFU negative regulator of hedgehog signaling |
| Gene type | protein-coding |
| Also known as | JBTS32, PRO1280, SUFUH, SUFUXL |
| Summary | The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SUFU-202 | ENST00000369902.8 | 5016 | 484aa | Protein coding | CCDS7537 | Q9UMX1-1 | NM_016169.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| SUFU-201 | ENST00000369899.6 | 1839 | 433aa | Protein coding | CCDS53571 | Q9UMX1-2 | - | TSL:1, GENCODE basic, |
| SUFU-203 | ENST00000423559.2 | 2601 | 481aa | Protein coding | - | Q9UMX1-3 | - | TSL:1, GENCODE basic, |
| SUFU-204 | ENST00000471000.1 | 803 | No protein | Processed transcript | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SUFU |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SUFU had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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