CRISPR-U™ Gene Knockout Cell Line Strategy

SUFU Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human SUFU Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SUFU
Gene id 51684
Organism Homo sapiens
Official full symbol SUFU negative regulator of hedgehog signaling
Gene type protein-coding
Also known as JBTS32, PRO1280, SUFUH, SUFUXL
Summary The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 10
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SUFU-202 ENST00000369902.8 5016 484aa Protein coding CCDS7537 Q9UMX1-1 NM_016169.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
SUFU-201 ENST00000369899.6 1839 433aa Protein coding CCDS53571 Q9UMX1-2 - TSL:1, GENCODE basic,
SUFU-203 ENST00000423559.2 2601 481aa Protein coding - Q9UMX1-3 - TSL:1, GENCODE basic,
SUFU-204 ENST00000471000.1 803 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SUFU
This KO Strategy loading
Red Cotton™ Notes Gene SUFU had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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