CRISPR-U™ Gene Knockout Cell Line Strategy

PDK4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PDK4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PDK4
Gene id 5166
Organism Homo sapiens
Official full symbol pyruvate dehydrogenase kinase 4
Gene type protein-coding
Summary This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.
Genomic regions Chromosome 7
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PDK4-201 ENST00000005178.6 3601 411aa Protein coding CCDS5643 A4D1H4 Q16654 NM_002612.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PDK4-204 ENST00000473796.1 2136 No protein Retained intron - - - TSL:2,
PDK4-203 ENST00000473301.1 691 No protein Retained intron - - - TSL:2,
PDK4-202 ENST00000468445.1 572 No protein Retained intron - - - TSL:4,
PDK4-205 ENST00000498190.1 420 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PDK4
This KO Strategy loading
Red Cotton™ Notes Gene PDK4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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