CRISPR-U™ Gene Knockout Cell Line
Strategy
PDK4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PDK4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PDK4 |
| Gene id | 5166 |
| Organism | Homo sapiens |
| Official full symbol | pyruvate dehydrogenase kinase 4 |
| Gene type | protein-coding |
| Summary | This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PDK4-201 | ENST00000005178.6 | 3601 | 411aa | Protein coding | CCDS5643 | A4D1H4 Q16654 | NM_002612.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PDK4-204 | ENST00000473796.1 | 2136 | No protein | Retained intron | - | - | - | TSL:2, |
| PDK4-203 | ENST00000473301.1 | 691 | No protein | Retained intron | - | - | - | TSL:2, |
| PDK4-202 | ENST00000468445.1 | 572 | No protein | Retained intron | - | - | - | TSL:4, |
| PDK4-205 | ENST00000498190.1 | 420 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PDK4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PDK4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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