CRISPR-U™ Gene Knockout Cell Line Strategy

PTRH2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PTRH2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PTRH2
Gene id 51651
Organism Homo sapiens
Official full symbol peptidyl-tRNA hydrolase 2
Gene type protein-coding
Also known as BIT1, CFAP37, CGI-147, IMNEPD, PTH, PTH 2, PTH2
Summary The protein encoded by this gene is a mitochondrial protein with two putative domains, an N-terminal mitochondrial localization sequence, and a UPF0099 domain. In vitro assays suggest that this protein possesses peptidyl-tRNA hydrolase activity, to release the peptidyl moiety from tRNA, thereby preventing the accumulation of dissociated peptidyl-tRNA that could reduce the efficiency of translation. This protein also plays a role regulating cell survival and death. It promotes survival as part of an integrin-signaling pathway for cells attached to the extracellular matrix (ECM), but also promotes apoptosis in cells that have lost their attachment to the ECM, a process called anoikos. After loss of cell attachment to the ECM, this protein is phosphorylated, is released from the mitochondria into the cytosol, and promotes caspase-independent apoptosis through interactions with transcriptional regulators. This gene has been implicated in the development and progression of tumors, and mutations in this gene have been associated with an infantile multisystem neurologic, endocrine, and pancreatic disease (INMEPD) characterized by intellectual disability, postnatal microcephaly, progressive cerebellar atrophy, hearing impairment, polyneuropathy, failure to thrive, and organ fibrosis with exocrine pancreas insufficiency (PMID: 25574476). Alternative splicing results in multiple transcript variants encoding different isoforms.
Genomic regions Chromosome 17
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PTRH2-203 ENST00000470557.2 4114 179aa Protein coding CCDS11618 Q9Y3E5 - TSL:NA, GENCODE basic, APPRIS ALT1,
PTRH2-202 ENST00000409433.2 2246 180aa Protein coding CCDS77076 J3KQ48 - TSL:1, GENCODE basic, APPRIS P4,
PTRH2-201 ENST00000393038.3 731 179aa Protein coding CCDS11618 Q9Y3E5 NM_016077.5 TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
PTRH2-204 ENST00000579915.1 531 No protein Processed transcript - - - TSL:4,
PTRH2-205 ENST00000587935.1 409 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PTRH2
This KO Strategy loading
Red Cotton™ Notes Gene PTRH2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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