CRISPR-U™ Gene Knockout Cell Line
Strategy
PTRH2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PTRH2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PTRH2 |
| Gene id | 51651 |
| Organism | Homo sapiens |
| Official full symbol | peptidyl-tRNA hydrolase 2 |
| Gene type | protein-coding |
| Also known as | BIT1, CFAP37, CGI-147, IMNEPD, PTH, PTH 2, PTH2 |
| Summary | The protein encoded by this gene is a mitochondrial protein with two putative domains, an N-terminal mitochondrial localization sequence, and a UPF0099 domain. In vitro assays suggest that this protein possesses peptidyl-tRNA hydrolase activity, to release the peptidyl moiety from tRNA, thereby preventing the accumulation of dissociated peptidyl-tRNA that could reduce the efficiency of translation. This protein also plays a role regulating cell survival and death. It promotes survival as part of an integrin-signaling pathway for cells attached to the extracellular matrix (ECM), but also promotes apoptosis in cells that have lost their attachment to the ECM, a process called anoikos. After loss of cell attachment to the ECM, this protein is phosphorylated, is released from the mitochondria into the cytosol, and promotes caspase-independent apoptosis through interactions with transcriptional regulators. This gene has been implicated in the development and progression of tumors, and mutations in this gene have been associated with an infantile multisystem neurologic, endocrine, and pancreatic disease (INMEPD) characterized by intellectual disability, postnatal microcephaly, progressive cerebellar atrophy, hearing impairment, polyneuropathy, failure to thrive, and organ fibrosis with exocrine pancreas insufficiency (PMID: 25574476). Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PTRH2-203 | ENST00000470557.2 | 4114 | 179aa | Protein coding | CCDS11618 | Q9Y3E5 | - | TSL:NA, GENCODE basic, APPRIS ALT1, |
| PTRH2-202 | ENST00000409433.2 | 2246 | 180aa | Protein coding | CCDS77076 | J3KQ48 | - | TSL:1, GENCODE basic, APPRIS P4, |
| PTRH2-201 | ENST00000393038.3 | 731 | 179aa | Protein coding | CCDS11618 | Q9Y3E5 | NM_016077.5 | TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| PTRH2-204 | ENST00000579915.1 | 531 | No protein | Processed transcript | - | - | - | TSL:4, |
| PTRH2-205 | ENST00000587935.1 | 409 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PTRH2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PTRH2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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