CRISPR-U™ Gene Knockout Cell Line
Strategy
PDK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PDK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PDK1 |
| Gene id | 5163 |
| Organism | Homo sapiens |
| Official full symbol | pyruvate dehydrogenase kinase 1 |
| Gene type | protein-coding |
| Summary | Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PDK1-201 | ENST00000282077.8 | 14072 | 436aa | Protein coding | CCDS2250 | Q15118-1 | NM_002610.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PDK1-203 | ENST00000410055.5 | 2990 | 436aa | Protein coding | CCDS2250 | Q15118-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PDK1-202 | ENST00000392571.6 | 1515 | 456aa | Protein coding | CCDS63059 | Q15118-2 | - | TSL:1, GENCODE basic, |
| PDK1-208 | ENST00000443353.5 | 842 | 154aa | Protein coding | - | C9JKT3 | - | CDS 3' incomplete, TSL:2, |
| PDK1-204 | ENST00000416991.5 | 699 | 229aa | Protein coding | - | C9IYB4 | - | CDS 3' incomplete, TSL:5, |
| PDK1-207 | ENST00000439519.1 | 558 | 154aa | Protein coding | - | C9JKT3 | - | CDS 3' incomplete, TSL:4, |
| PDK1-205 | ENST00000431718.5 | 728 | 164aa | Nonsense mediated decay | - | F8WEJ6 | - | TSL:3, |
| PDK1-206 | ENST00000436490.5 | 571 | 62aa | Nonsense mediated decay | - | F8WC75 | - | TSL:5, |
| PDK1-209 | ENST00000466437.1 | 599 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PDK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PDK1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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