CRISPR-U™ Gene Knockout Cell Line Strategy

PDK1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PDK1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PDK1
Gene id 5163
Organism Homo sapiens
Official full symbol pyruvate dehydrogenase kinase 1
Gene type protein-coding
Summary Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene.
Genomic regions Chromosome 2
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PDK1-201 ENST00000282077.8 14072 436aa Protein coding CCDS2250 Q15118-1 NM_002610.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PDK1-203 ENST00000410055.5 2990 436aa Protein coding CCDS2250 Q15118-1 - TSL:1, GENCODE basic, APPRIS P1,
PDK1-202 ENST00000392571.6 1515 456aa Protein coding CCDS63059 Q15118-2 - TSL:1, GENCODE basic,
PDK1-208 ENST00000443353.5 842 154aa Protein coding - C9JKT3 - CDS 3' incomplete, TSL:2,
PDK1-204 ENST00000416991.5 699 229aa Protein coding - C9IYB4 - CDS 3' incomplete, TSL:5,
PDK1-207 ENST00000439519.1 558 154aa Protein coding - C9JKT3 - CDS 3' incomplete, TSL:4,
PDK1-205 ENST00000431718.5 728 164aa Nonsense mediated decay - F8WEJ6 - TSL:3,
PDK1-206 ENST00000436490.5 571 62aa Nonsense mediated decay - F8WC75 - TSL:5,
PDK1-209 ENST00000466437.1 599 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PDK1
This KO Strategy loading
Red Cotton™ Notes Gene PDK1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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