CRISPR-U™ Gene Knockout Cell Line
Strategy
ERGIC3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ERGIC3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ERGIC3 |
| Gene id | 51614 |
| Organism | Homo sapiens |
| Official full symbol | ERGIC and golgi 3 |
| Gene type | protein-coding |
| Also known as | C20orf47, C2orf47, CGI-54, Erv46, NY-BR-84, PRO0989, SDBCAG84, dJ477O4.2 |
| Genomic regions | Chromosome 20 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ERGIC3-203 | ENST00000357394.8 | 1357 | 388aa | Protein coding | CCDS13258 | Q9Y282-3 | - | TSL:2, GENCODE basic, APPRIS ALT1, |
| ERGIC3-202 | ENST00000348547.7 | 1302 | 383aa | Protein coding | CCDS13257 | Q9Y282-1 | NM_015966.3 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| ERGIC3-206 | ENST00000416206.5 | 1235 | 397aa | Protein coding | - | H0Y5K5 | - | CDS 5' incomplete, TSL:5, |
| ERGIC3-205 | ENST00000413587.5 | 977 | 326aa | Protein coding | - | H0Y621 | - | CDS 5' and 3' incomplete, TSL:5, |
| ERGIC3-220 | ENST00000640748.1 | 832 | 198aa | Protein coding | - | A0A1W2PPS8 | - | TSL:5, GENCODE basic, |
| ERGIC3-204 | ENST00000411577.5 | 601 | 118aa | Protein coding | - | A6PVJ2 | - | CDS 5' incomplete, TSL:2, |
| ERGIC3-210 | ENST00000451605.1 | 520 | 130aa | Protein coding | - | H0Y802 | - | CDS 5' incomplete, TSL:2, |
| ERGIC3-208 | ENST00000442139.5 | 472 | 157aa | Protein coding | - | H0Y6Z0 | - | CDS 5' and 3' incomplete, TSL:3, |
| ERGIC3-207 | ENST00000438317.5 | 590 | 123aa | Nonsense mediated decay | - | A0A0C4DH46 | - | TSL:3, |
| ERGIC3-214 | ENST00000482338.5 | 598 | No protein | Processed transcript | - | - | - | TSL:2, |
| ERGIC3-215 | ENST00000486268.5 | 584 | No protein | Processed transcript | - | - | - | TSL:2, |
| ERGIC3-218 | ENST00000489071.5 | 582 | No protein | Processed transcript | - | - | - | TSL:2, |
| ERGIC3-217 | ENST00000488935.5 | 389 | No protein | Processed transcript | - | - | - | TSL:2, |
| ERGIC3-201 | ENST00000279052.10 | 2846 | No protein | Retained intron | - | - | - | TSL:2, |
| ERGIC3-209 | ENST00000447986.5 | 1632 | No protein | Retained intron | - | - | - | TSL:1, |
| ERGIC3-219 | ENST00000496172.5 | 781 | No protein | Retained intron | - | - | - | TSL:2, |
| ERGIC3-216 | ENST00000488384.1 | 766 | No protein | Retained intron | - | - | - | TSL:2, |
| ERGIC3-211 | ENST00000461043.6 | 728 | No protein | Retained intron | - | - | - | TSL:2, |
| ERGIC3-213 | ENST00000475211.1 | 514 | No protein | Retained intron | - | - | - | TSL:3, |
| ERGIC3-212 | ENST00000465298.1 | 505 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ERGIC3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ERGIC3 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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