CRISPR-U™ Gene Knockout Cell Line Strategy

ERGIC3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ERGIC3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ERGIC3
Gene id 51614
Organism Homo sapiens
Official full symbol ERGIC and golgi 3
Gene type protein-coding
Also known as C20orf47, C2orf47, CGI-54, Erv46, NY-BR-84, PRO0989, SDBCAG84, dJ477O4.2
Genomic regions Chromosome 20
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ERGIC3-203 ENST00000357394.8 1357 388aa Protein coding CCDS13258 Q9Y282-3 - TSL:2, GENCODE basic, APPRIS ALT1,
ERGIC3-202 ENST00000348547.7 1302 383aa Protein coding CCDS13257 Q9Y282-1 NM_015966.3 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
ERGIC3-206 ENST00000416206.5 1235 397aa Protein coding - H0Y5K5 - CDS 5' incomplete, TSL:5,
ERGIC3-205 ENST00000413587.5 977 326aa Protein coding - H0Y621 - CDS 5' and 3' incomplete, TSL:5,
ERGIC3-220 ENST00000640748.1 832 198aa Protein coding - A0A1W2PPS8 - TSL:5, GENCODE basic,
ERGIC3-204 ENST00000411577.5 601 118aa Protein coding - A6PVJ2 - CDS 5' incomplete, TSL:2,
ERGIC3-210 ENST00000451605.1 520 130aa Protein coding - H0Y802 - CDS 5' incomplete, TSL:2,
ERGIC3-208 ENST00000442139.5 472 157aa Protein coding - H0Y6Z0 - CDS 5' and 3' incomplete, TSL:3,
ERGIC3-207 ENST00000438317.5 590 123aa Nonsense mediated decay - A0A0C4DH46 - TSL:3,
ERGIC3-214 ENST00000482338.5 598 No protein Processed transcript - - - TSL:2,
ERGIC3-215 ENST00000486268.5 584 No protein Processed transcript - - - TSL:2,
ERGIC3-218 ENST00000489071.5 582 No protein Processed transcript - - - TSL:2,
ERGIC3-217 ENST00000488935.5 389 No protein Processed transcript - - - TSL:2,
ERGIC3-201 ENST00000279052.10 2846 No protein Retained intron - - - TSL:2,
ERGIC3-209 ENST00000447986.5 1632 No protein Retained intron - - - TSL:1,
ERGIC3-219 ENST00000496172.5 781 No protein Retained intron - - - TSL:2,
ERGIC3-216 ENST00000488384.1 766 No protein Retained intron - - - TSL:2,
ERGIC3-211 ENST00000461043.6 728 No protein Retained intron - - - TSL:2,
ERGIC3-213 ENST00000475211.1 514 No protein Retained intron - - - TSL:3,
ERGIC3-212 ENST00000465298.1 505 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ERGIC3
This KO Strategy loading
Red Cotton™ Notes Gene ERGIC3 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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