CRISPR-U™ Gene Knockout Cell Line
Strategy
PIGT Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PIGT Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PIGT |
| Gene id | 51604 |
| Organism | Homo sapiens |
| Official full symbol | phosphatidylinositol glycan anchor biosynthesis class T |
| Gene type | protein-coding |
| Also known as | CGI-06, MCAHS3, NDAP, PNH2 |
| Summary | This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Genomic regions | Chromosome 20 |
Strategy Summary
This gene has 19 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PIGT-202 | ENST00000279036.12 | 2168 | 578aa | Protein coding | CCDS13353 | Q969N2-1 | NM_015937.6 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| PIGT-207 | ENST00000543458.7 | 2037 | 522aa | Protein coding | CCDS54464 | Q969N2-5 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| PIGT-203 | ENST00000372689.9 | 1971 | 511aa | Protein coding | CCDS54465 | Q969N2-6 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PIGT-201 | ENST00000279035.14 | 1880 | 476aa | Protein coding | CCDS54466 | Q969N2-4 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| PIGT-260 | ENST00000640324.1 | 2098 | 580aa | Protein coding | - | A0A1W2PNP0 | - | CDS 5' incomplete, TSL:5, |
| PIGT-246 | ENST00000639499.1 | 2021 | 495aa | Protein coding | - | A0A1W2PP57 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| PIGT-243 | ENST00000639292.1 | 1938 | 532aa | Protein coding | - | A0A1W2PPR6 | - | CDS 5' incomplete, TSL:5, |
| PIGT-221 | ENST00000638594.1 | 1835 | 505aa | Protein coding | - | A0A1W2PPS0 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| PIGT-257 | ENST00000640210.1 | 1715 | 441aa | Protein coding | - | A0A1W2PP53 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| PIGT-244 | ENST00000639382.1 | 1193 | 393aa | Protein coding | - | A0A1W2PPQ7 | - | CDS 3' incomplete, TSL:1, |
| PIGT-208 | ENST00000545755.3 | 1158 | 384aa | Protein coding | - | Q969N2-2 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| PIGT-218 | ENST00000638478.1 | 1076 | 354aa | Protein coding | - | A0A1W2PP13 | - | CDS 3' incomplete, TSL:3, |
| PIGT-213 | ENST00000638353.1 | 970 | 318aa | Protein coding | - | A0A1W2PQ52 | - | CDS 3' incomplete, TSL:1, |
| PIGT-239 | ENST00000639235.1 | 961 | 316aa | Protein coding | - | A0A1W2PRH2 | - | CDS 3' incomplete, TSL:1, |
| PIGT-219 | ENST00000638489.1 | 957 | 314aa | Protein coding | - | A0A1W2PPI8 | - | CDS 3' incomplete, TSL:1, |
| PIGT-254 | ENST00000640123.1 | 847 | 61aa | Protein coding | - | A0A1W2PR34 | - | CDS 5' incomplete, TSL:5, |
| PIGT-205 | ENST00000432270.2 | 832 | 275aa | Protein coding | - | H7C2Y2 | - | CDS 3' incomplete, TSL:3, |
| PIGT-226 | ENST00000638691.1 | 823 | 136aa | Protein coding | - | A0A1W2PR73 | - | CDS 5' incomplete, TSL:3, |
| PIGT-240 | ENST00000639239.1 | 505 | 128aa | Protein coding | - | A0A1W2PSC5 | - | CDS 5' incomplete, TSL:2, |
| PIGT-229 | ENST00000638745.1 | 6087 | 145aa | Nonsense mediated decay | - | A0A1W2PQI9 | - | TSL:5, |
| PIGT-267 | ENST00000640666.1 | 2502 | 499aa | Nonsense mediated decay | - | A0A1W2PPC3 | - | TSL:5, |
| PIGT-227 | ENST00000638710.1 | 2327 | 77aa | Nonsense mediated decay | - | A0A1W2PP91 | - | CDS 5' incomplete, TSL:5, |
| PIGT-222 | ENST00000638612.1 | 2311 | 499aa | Nonsense mediated decay | - | A0A1W2PPC3 | - | TSL:5, |
| PIGT-214 | ENST00000638383.1 | 2235 | 232aa | Nonsense mediated decay | - | A0A1W2PRG1 | - | TSL:5, |
| PIGT-272 | ENST00000640996.1 | 2219 | 140aa | Nonsense mediated decay | - | A0A1W2PNZ5 | - | TSL:5, |
| PIGT-264 | ENST00000640585.1 | 2203 | 140aa | Nonsense mediated decay | - | A0A1W2PNZ5 | - | TSL:5, |
| PIGT-271 | ENST00000640986.1 | 2187 | 300aa | Nonsense mediated decay | - | A0A1W2PQ29 | - | TSL:2, |
| PIGT-215 | ENST00000638387.1 | 2170 | 331aa | Nonsense mediated decay | - | A0A1W2PRV7 | - | TSL:5, |
| PIGT-262 | ENST00000640542.1 | 2169 | 432aa | Nonsense mediated decay | - | A0A1W2PR92 | - | TSL:5, |
| PIGT-250 | ENST00000639932.1 | 2158 | 242aa | Nonsense mediated decay | - | A0A1W2PPU8 | - | TSL:5, |
| PIGT-259 | ENST00000640272.1 | 2112 | 203aa | Nonsense mediated decay | - | A0A1W2PRH8 | - | TSL:5, |
| PIGT-253 | ENST00000640108.1 | 2109 | 116aa | Nonsense mediated decay | - | A0A1W2PR22 | - | TSL:5, |
| PIGT-252 | ENST00000640107.1 | 2105 | 240aa | Nonsense mediated decay | - | A0A1W2PNW9 | - | TSL:5, |
| PIGT-236 | ENST00000638978.1 | 2091 | 429aa | Nonsense mediated decay | - | A0A1W2PRZ8 | - | TSL:5, |
| PIGT-217 | ENST00000638445.1 | 2088 | 191aa | Nonsense mediated decay | - | A0A1W2PP98 | - | TSL:2, |
| PIGT-204 | ENST00000424705.2 | 2046 | 78aa | Nonsense mediated decay | - | A0A1W2PQA4 | - | CDS 5' incomplete, TSL:5, |
| PIGT-211 | ENST00000638246.1 | 2032 | 123aa | Nonsense mediated decay | - | B7Z7C5 | - | TSL:5, |
| PIGT-232 | ENST00000638938.1 | 1985 | 140aa | Nonsense mediated decay | - | A0A1W2PNZ5 | - | TSL:5, |
| PIGT-251 | ENST00000639984.1 | 1965 | 171aa | Nonsense mediated decay | - | A0A1W2PRL6 | - | TSL:5, |
| PIGT-269 | ENST00000640692.1 | 1948 | 300aa | Nonsense mediated decay | - | A0A1W2PQ29 | - | TSL:5, |
| PIGT-224 | ENST00000638671.1 | 1944 | 145aa | Nonsense mediated decay | - | A0A1W2PQI9 | - | TSL:2, |
| PIGT-228 | ENST00000638714.1 | 1886 | 128aa | Nonsense mediated decay | - | A0A1W2PP03 | - | TSL:5, |
| PIGT-256 | ENST00000640194.1 | 1810 | 123aa | Nonsense mediated decay | - | B7Z7C5 | - | TSL:5, |
| PIGT-206 | ENST00000455050.2 | 1807 | 123aa | Nonsense mediated decay | - | B7Z7C5 | - | TSL:2, |
| PIGT-216 | ENST00000638415.1 | 1738 | 12aa | Nonsense mediated decay | - | A0A1W2PQ50 | - | CDS 5' incomplete, TSL:5, |
| PIGT-248 | ENST00000639783.1 | 1691 | 198aa | Nonsense mediated decay | - | A0A1W2PQL3 | - | TSL:2, |
| PIGT-231 | ENST00000638927.1 | 1457 | 213aa | Nonsense mediated decay | - | A0A1W2PQY1 | - | CDS 5' incomplete, TSL:5, |
| PIGT-263 | ENST00000640549.1 | 1437 | 329aa | Nonsense mediated decay | - | A0A1W2PQN1 | - | CDS 5' incomplete, TSL:5, |
| PIGT-255 | ENST00000640175.1 | 1429 | 96aa | Nonsense mediated decay | - | A0A1W2PRB4 | - | TSL:2, |
| PIGT-233 | ENST00000638953.1 | 952 | 140aa | Nonsense mediated decay | - | A0A1W2PNZ5 | - | TSL:5, |
| PIGT-245 | ENST00000639417.1 | 880 | 183aa | Nonsense mediated decay | - | A0A1W2PNS2 | - | TSL:3, |
| PIGT-212 | ENST00000638277.1 | 879 | 144aa | Nonsense mediated decay | - | A0A1W2PPK4 | - | CDS 5' incomplete, TSL:5, |
| PIGT-242 | ENST00000639286.1 | 861 | 101aa | Nonsense mediated decay | - | A0A1W2PR70 | - | TSL:5, |
| PIGT-230 | ENST00000638864.1 | 473 | 3aa | Nonsense mediated decay | - | - | - | CDS 5' incomplete, TSL:3, |
| PIGT-238 | ENST00000639194.1 | 439 | 18aa | Nonsense mediated decay | - | A0A1W2PQL9 | - | CDS 5' incomplete, TSL:5, |
| PIGT-268 | ENST00000640691.1 | 2447 | No protein | Processed transcript | - | - | - | TSL:5, |
| PIGT-220 | ENST00000638537.1 | 1902 | No protein | Processed transcript | - | - | - | TSL:5, |
| PIGT-270 | ENST00000640940.1 | 1764 | No protein | Processed transcript | - | - | - | TSL:5, |
| PIGT-237 | ENST00000639058.1 | 569 | No protein | Processed transcript | - | - | - | TSL:4, |
| PIGT-225 | ENST00000638689.1 | 4314 | No protein | Retained intron | - | - | - | TSL:5, |
| PIGT-241 | ENST00000639250.1 | 3363 | No protein | Retained intron | - | - | - | TSL:5, |
| PIGT-209 | ENST00000595203.1 | 3150 | No protein | Retained intron | - | - | - | TSL:NA, |
| PIGT-261 | ENST00000640364.1 | 2840 | No protein | Retained intron | - | - | - | TSL:5, |
| PIGT-235 | ENST00000638967.1 | 2194 | No protein | Retained intron | - | - | - | TSL:5, |
| PIGT-223 | ENST00000638641.1 | 2164 | No protein | Retained intron | - | - | - | TSL:NA, |
| PIGT-234 | ENST00000638962.1 | 2090 | No protein | Retained intron | - | - | - | TSL:5, |
| PIGT-210 | ENST00000638241.1 | 2006 | No protein | Retained intron | - | - | - | TSL:2, |
| PIGT-247 | ENST00000639664.1 | 1878 | No protein | Retained intron | - | - | - | TSL:5, |
| PIGT-249 | ENST00000639872.1 | 1498 | No protein | Retained intron | - | - | - | TSL:2, |
| PIGT-258 | ENST00000640253.1 | 1418 | No protein | Retained intron | - | - | - | TSL:2, |
| PIGT-265 | ENST00000640638.1 | 1196 | No protein | Retained intron | - | - | - | TSL:2, |
| PIGT-266 | ENST00000640649.1 | 536 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PIGT |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PIGT had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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Work flow
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