CRISPR-U™ Gene Knockout Cell Line
Strategy
AZIN1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human AZIN1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | AZIN1 |
| Gene id | 51582 |
| Organism | Homo sapiens |
| Official full symbol | antizyme inhibitor 1 |
| Gene type | protein-coding |
| Also known as | AZI, AZI1, AZIA1, OAZI, OAZIN, ODC1L |
| Summary | The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 1, the first member of this gene family that is ubiquitously expressed, and is localized in the nucleus and cytoplasm. Overexpression of antizyme inhibitor 1 gene has been associated with increased proliferation, cellular transformation and tumorigenesis. Gene knockout studies showed that homozygous mutant mice lacking functional antizyme inhibitor 1 gene died at birth with abnormal liver morphology. RNA editing of this gene, predominantly in the liver tissue, has been linked to the progression of hepatocellular carcinoma. Alternatively spliced transcript variants have been described for this gene. |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| AZIN1-201 | ENST00000337198.10 | 4196 | 448aa | Protein coding | CCDS6295 | A0A024R9C7 O14977 | NM_148174.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| AZIN1-202 | ENST00000347770.8 | 2857 | 448aa | Protein coding | CCDS6295 | A0A024R9C7 O14977 | - | TSL:1, GENCODE basic, APPRIS P1, |
| AZIN1-204 | ENST00000518353.5 | 773 | 33aa | Protein coding | - | E5RJ16 | - | CDS 3' incomplete, TSL:5, |
| AZIN1-208 | ENST00000521536.6 | 680 | 165aa | Protein coding | - | U3KQI8 | - | CDS 3' incomplete, TSL:5, |
| AZIN1-207 | ENST00000520402.5 | 660 | 97aa | Protein coding | - | E5RIB7 | - | CDS 3' incomplete, TSL:4, |
| AZIN1-209 | ENST00000522311.1 | 872 | No protein | Processed transcript | - | - | - | TSL:3, |
| AZIN1-203 | ENST00000517581.1 | 576 | No protein | Processed transcript | - | - | - | TSL:4, |
| AZIN1-206 | ENST00000518940.5 | 574 | No protein | Processed transcript | - | - | - | TSL:3, |
| AZIN1-205 | ENST00000518697.1 | 565 | No protein | Processed transcript | - | - | - | TSL:4, |
| AZIN1-210 | ENST00000523071.1 | 839 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | AZIN1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene AZIN1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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