CRISPR-U™ Gene Knockout Cell Line Strategy

AZIN1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human AZIN1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol AZIN1
Gene id 51582
Organism Homo sapiens
Official full symbol antizyme inhibitor 1
Gene type protein-coding
Also known as AZI, AZI1, AZIA1, OAZI, OAZIN, ODC1L
Summary The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 1, the first member of this gene family that is ubiquitously expressed, and is localized in the nucleus and cytoplasm. Overexpression of antizyme inhibitor 1 gene has been associated with increased proliferation, cellular transformation and tumorigenesis. Gene knockout studies showed that homozygous mutant mice lacking functional antizyme inhibitor 1 gene died at birth with abnormal liver morphology. RNA editing of this gene, predominantly in the liver tissue, has been linked to the progression of hepatocellular carcinoma. Alternatively spliced transcript variants have been described for this gene.
Genomic regions Chromosome 8
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
AZIN1-201 ENST00000337198.10 4196 448aa Protein coding CCDS6295 A0A024R9C7 O14977 NM_148174.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
AZIN1-202 ENST00000347770.8 2857 448aa Protein coding CCDS6295 A0A024R9C7 O14977 - TSL:1, GENCODE basic, APPRIS P1,
AZIN1-204 ENST00000518353.5 773 33aa Protein coding - E5RJ16 - CDS 3' incomplete, TSL:5,
AZIN1-208 ENST00000521536.6 680 165aa Protein coding - U3KQI8 - CDS 3' incomplete, TSL:5,
AZIN1-207 ENST00000520402.5 660 97aa Protein coding - E5RIB7 - CDS 3' incomplete, TSL:4,
AZIN1-209 ENST00000522311.1 872 No protein Processed transcript - - - TSL:3,
AZIN1-203 ENST00000517581.1 576 No protein Processed transcript - - - TSL:4,
AZIN1-206 ENST00000518940.5 574 No protein Processed transcript - - - TSL:3,
AZIN1-205 ENST00000518697.1 565 No protein Processed transcript - - - TSL:4,
AZIN1-210 ENST00000523071.1 839 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene AZIN1
This KO Strategy loading
Red Cotton™ Notes Gene AZIN1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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