CRISPR-U™ Gene Knockout Cell Line
Strategy
SIRT6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SIRT6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SIRT6 |
| Gene id | 51548 |
| Organism | Homo sapiens |
| Official full symbol | sirtuin 6 |
| Gene type | protein-coding |
| Also known as | SIR2L6 |
| Summary | This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SIRT6-202 | ENST00000337491.7 | 1600 | 355aa | Protein coding | CCDS12122 | Q8N6T7-1 | NM_016539.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| SIRT6-203 | ENST00000594279.5 | 1507 | 176aa | Protein coding | CCDS82275 | M0R1N9 | - | TSL:3, GENCODE basic, |
| SIRT6-201 | ENST00000305232.10 | 1506 | 328aa | Protein coding | CCDS54199 | Q8N6T7-2 | - | TSL:1, GENCODE basic, |
| SIRT6-214 | ENST00000601488.5 | 1361 | 187aa | Protein coding | CCDS82276 | M0QXA0 | - | TSL:3, GENCODE basic, |
| SIRT6-208 | ENST00000597896.5 | 827 | 275aa | Protein coding | - | M0QZ09 | - | CDS 5' and 3' incomplete, TSL:3, |
| SIRT6-215 | ENST00000601571.1 | 716 | 186aa | Protein coding | - | M0R1F6 | - | CDS 5' incomplete, TSL:2, |
| SIRT6-212 | ENST00000600938.5 | 1473 | 43aa | Nonsense mediated decay | - | M0R0B2 | - | TSL:2, |
| SIRT6-209 | ENST00000599365.5 | 1372 | 43aa | Nonsense mediated decay | - | M0R0B2 | - | TSL:1, |
| SIRT6-206 | ENST00000596119.5 | 1537 | No protein | Retained intron | - | - | - | TSL:5, |
| SIRT6-211 | ENST00000600540.5 | 1407 | No protein | Retained intron | - | - | - | TSL:2, |
| SIRT6-213 | ENST00000601069.5 | 1260 | No protein | Retained intron | - | - | - | TSL:2, |
| SIRT6-205 | ENST00000595670.5 | 1057 | No protein | Retained intron | - | - | - | TSL:5, |
| SIRT6-207 | ENST00000596298.5 | 717 | No protein | Retained intron | - | - | - | TSL:2, |
| SIRT6-210 | ENST00000599394.1 | 607 | No protein | Retained intron | - | - | - | TSL:4, |
| SIRT6-204 | ENST00000594341.1 | 371 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SIRT6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SIRT6 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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