CRISPR-U™ Gene Knockout Cell Line Strategy

SIRT6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SIRT6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SIRT6
Gene id 51548
Organism Homo sapiens
Official full symbol sirtuin 6
Gene type protein-coding
Also known as SIR2L6
Summary This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms.
Genomic regions Chromosome 19
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SIRT6-202 ENST00000337491.7 1600 355aa Protein coding CCDS12122 Q8N6T7-1 NM_016539.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
SIRT6-203 ENST00000594279.5 1507 176aa Protein coding CCDS82275 M0R1N9 - TSL:3, GENCODE basic,
SIRT6-201 ENST00000305232.10 1506 328aa Protein coding CCDS54199 Q8N6T7-2 - TSL:1, GENCODE basic,
SIRT6-214 ENST00000601488.5 1361 187aa Protein coding CCDS82276 M0QXA0 - TSL:3, GENCODE basic,
SIRT6-208 ENST00000597896.5 827 275aa Protein coding - M0QZ09 - CDS 5' and 3' incomplete, TSL:3,
SIRT6-215 ENST00000601571.1 716 186aa Protein coding - M0R1F6 - CDS 5' incomplete, TSL:2,
SIRT6-212 ENST00000600938.5 1473 43aa Nonsense mediated decay - M0R0B2 - TSL:2,
SIRT6-209 ENST00000599365.5 1372 43aa Nonsense mediated decay - M0R0B2 - TSL:1,
SIRT6-206 ENST00000596119.5 1537 No protein Retained intron - - - TSL:5,
SIRT6-211 ENST00000600540.5 1407 No protein Retained intron - - - TSL:2,
SIRT6-213 ENST00000601069.5 1260 No protein Retained intron - - - TSL:2,
SIRT6-205 ENST00000595670.5 1057 No protein Retained intron - - - TSL:5,
SIRT6-207 ENST00000596298.5 717 No protein Retained intron - - - TSL:2,
SIRT6-210 ENST00000599394.1 607 No protein Retained intron - - - TSL:4,
SIRT6-204 ENST00000594341.1 371 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SIRT6
This KO Strategy loading
Red Cotton™ Notes Gene SIRT6 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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