CRISPR-U™ Gene Knockout Cell Line Strategy

JKAMP Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human JKAMP Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol JKAMP
Gene id 51528
Organism Homo sapiens
Official full symbol JNK1/MAPK8 associated membrane protein
Gene type protein-coding
Also known as C14orf100, C24orf100, CDA06, HSPC213, HSPC327, JAMP
Genomic regions Chromosome 14
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
JKAMP-201 ENST00000356057.9 2524 319aa Protein coding CCDS61463 Q9P055-5 - TSL:1, GENCODE basic,
JKAMP-214 ENST00000616435.5 2347 311aa Protein coding CCDS45116 Q9P055-4 NM_016475.5 TSL:5, GENCODE basic, APPRIS P4, MANE Select v0.92,
JKAMP-206 ENST00000554271.5 2137 325aa Protein coding CCDS61462 G3V2M4 - TSL:2, GENCODE basic,
JKAMP-202 ENST00000425728.6 1636 305aa Protein coding CCDS45117 Q9P055-3 - TSL:1, GENCODE basic, APPRIS ALT1,
JKAMP-209 ENST00000554795.1 856 247aa Protein coding - G3V2R2 - CDS 3' incomplete, TSL:5,
JKAMP-211 ENST00000556985.5 592 153aa Protein coding - G3V372 - TSL:2, GENCODE basic,
JKAMP-210 ENST00000555491.5 619 140aa Nonsense mediated decay - G3V4D0 - TSL:3,
JKAMP-212 ENST00000557560.5 611 No protein Processed transcript - - - TSL:4,
JKAMP-208 ENST00000554754.5 562 No protein Processed transcript - - - TSL:3,
JKAMP-213 ENST00000602482.5 2302 No protein Retained intron - - - TSL:1,
JKAMP-207 ENST00000554721.1 1951 No protein Retained intron - - - TSL:2,
JKAMP-205 ENST00000553941.5 1191 No protein Retained intron - - - TSL:2,
JKAMP-203 ENST00000553156.2 570 No protein Retained intron - - - TSL:4,
JKAMP-204 ENST00000553647.1 569 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene JKAMP
This KO Strategy loading
Red Cotton™ Notes Gene JKAMP had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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