CRISPR-U™ Gene Knockout Cell Line
Strategy
ETV7 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ETV7 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ETV7 |
| Gene id | 51513 |
| Organism | Homo sapiens |
| Official full symbol | ETS variant transcription factor 7 |
| Gene type | protein-coding |
| Also known as | TEL-2, TEL2, TELB |
| Summary | The protein encoded by this gene belongs to the ETS family of transcription factors, which is a large group of evolutionarily conserved transcriptional regulators that play an important role in a variety of cellular processes throughout development and differentiation, and are involved in oncogenesis as well. This protein is predominantly expressed in hematopoietic tissues. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene (PMID:11108721). |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ETV7-206 | ENST00000615781.4 | 1776 | 282aa | Protein coding | CCDS75440 | Q9Y603-2 | - | TSL:1, GENCODE basic, |
| ETV7-202 | ENST00000340181.9 | 1605 | 341aa | Protein coding | CCDS4819 | Q9Y603-1 | NM_016135.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| ETV7-207 | ENST00000620358.4 | 1599 | 260aa | Protein coding | CCDS75441 | Q9Y603-3 | - | TSL:1, GENCODE basic, |
| ETV7-204 | ENST00000373738.4 | 1570 | 286aa | Protein coding | CCDS56423 | Q9Y603-4 | - | TSL:1, GENCODE basic, |
| ETV7-201 | ENST00000339796.9 | 1530 | 317aa | Protein coding | CCDS56425 | Q9Y603-5 | - | TSL:1, GENCODE basic, |
| ETV7-203 | ENST00000373737.8 | 1488 | 264aa | Protein coding | CCDS56424 | Q9Y603-7 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| ETV7-205 | ENST00000538992.3 | 1434 | 190aa | Protein coding | CCDS56422 | Q9Y603-8 | - | TSL:2, GENCODE basic, |
| ETV7-208 | ENST00000627426.2 | 1285 | 262aa | Protein coding | CCDS78131 | Q9Y603-6 | - | TSL:1, GENCODE basic, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ETV7 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ETV7 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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