CRISPR-U™ Gene Knockout Cell Line Strategy

PDE4B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PDE4B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PDE4B
Gene id 5142
Organism Homo sapiens
Official full symbol phosphodiesterase 4B
Gene type protein-coding
Also known as DPDE4, PDEIVB
Summary This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. The encoded protein regulates the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms.
Genomic regions Chromosome 1
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PDE4B-206 ENST00000423207.6 4546 721aa Protein coding CCDS30742 Q07343-3 - TSL:1, GENCODE basic, APPRIS P3,
PDE4B-202 ENST00000341517.9 4531 736aa Protein coding CCDS632 Q07343-1 NM_002600.4 TSL:1, APPRIS ALT1, MANE Select v0.92,
PDE4B-201 ENST00000329654.8 3998 736aa Protein coding CCDS632 Q07343-1 - TSL:1, GENCODE basic, APPRIS ALT1,
PDE4B-203 ENST00000371045.9 3982 564aa Protein coding CCDS30743 Q07343-2 - TSL:1, GENCODE basic,
PDE4B-207 ENST00000480109.2 2037 503aa Protein coding CCDS72802 Q07343-4 - TSL:1, GENCODE basic,
PDE4B-205 ENST00000412480.6 579 147aa Protein coding - E9PMG3 - CDS 3' incomplete, TSL:4,
PDE4B-215 ENST00000531025.5 561 143aa Protein coding - E9PNB0 - CDS 3' incomplete, TSL:4,
PDE4B-212 ENST00000526197.5 550 152aa Protein coding - E9PR34 - CDS 3' incomplete, TSL:4,
PDE4B-214 ENST00000528771.5 546 137aa Protein coding - E9PJ03 - CDS 3' incomplete, TSL:4,
PDE4B-210 ENST00000491340.2 226 66aa Protein coding - H0YCV7 - CDS 5' incomplete, TSL:3,
PDE4B-204 ENST00000371048.7 1136 No protein Processed transcript - - - TSL:3,
PDE4B-209 ENST00000490695.1 728 No protein Processed transcript - - - TSL:3,
PDE4B-211 ENST00000524999.1 615 No protein Processed transcript - - - TSL:4,
PDE4B-217 ENST00000532040.1 570 No protein Processed transcript - - - TSL:4,
PDE4B-213 ENST00000526666.1 558 No protein Processed transcript - - - TSL:4,
PDE4B-216 ENST00000531358.1 556 No protein Processed transcript - - - TSL:4,
PDE4B-218 ENST00000534463.5 866 No protein Retained intron - - - TSL:3,
PDE4B-208 ENST00000483863.2 572 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PDE4B
This KO Strategy loading
Red Cotton™ Notes Gene PDE4B had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×