CRISPR-U™ Gene Knockout Cell Line
Strategy
PDE4B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PDE4B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PDE4B |
| Gene id | 5142 |
| Organism | Homo sapiens |
| Official full symbol | phosphodiesterase 4B |
| Gene type | protein-coding |
| Also known as | DPDE4, PDEIVB |
| Summary | This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. The encoded protein regulates the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PDE4B-206 | ENST00000423207.6 | 4546 | 721aa | Protein coding | CCDS30742 | Q07343-3 | - | TSL:1, GENCODE basic, APPRIS P3, |
| PDE4B-202 | ENST00000341517.9 | 4531 | 736aa | Protein coding | CCDS632 | Q07343-1 | NM_002600.4 | TSL:1, APPRIS ALT1, MANE Select v0.92, |
| PDE4B-201 | ENST00000329654.8 | 3998 | 736aa | Protein coding | CCDS632 | Q07343-1 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| PDE4B-203 | ENST00000371045.9 | 3982 | 564aa | Protein coding | CCDS30743 | Q07343-2 | - | TSL:1, GENCODE basic, |
| PDE4B-207 | ENST00000480109.2 | 2037 | 503aa | Protein coding | CCDS72802 | Q07343-4 | - | TSL:1, GENCODE basic, |
| PDE4B-205 | ENST00000412480.6 | 579 | 147aa | Protein coding | - | E9PMG3 | - | CDS 3' incomplete, TSL:4, |
| PDE4B-215 | ENST00000531025.5 | 561 | 143aa | Protein coding | - | E9PNB0 | - | CDS 3' incomplete, TSL:4, |
| PDE4B-212 | ENST00000526197.5 | 550 | 152aa | Protein coding | - | E9PR34 | - | CDS 3' incomplete, TSL:4, |
| PDE4B-214 | ENST00000528771.5 | 546 | 137aa | Protein coding | - | E9PJ03 | - | CDS 3' incomplete, TSL:4, |
| PDE4B-210 | ENST00000491340.2 | 226 | 66aa | Protein coding | - | H0YCV7 | - | CDS 5' incomplete, TSL:3, |
| PDE4B-204 | ENST00000371048.7 | 1136 | No protein | Processed transcript | - | - | - | TSL:3, |
| PDE4B-209 | ENST00000490695.1 | 728 | No protein | Processed transcript | - | - | - | TSL:3, |
| PDE4B-211 | ENST00000524999.1 | 615 | No protein | Processed transcript | - | - | - | TSL:4, |
| PDE4B-217 | ENST00000532040.1 | 570 | No protein | Processed transcript | - | - | - | TSL:4, |
| PDE4B-213 | ENST00000526666.1 | 558 | No protein | Processed transcript | - | - | - | TSL:4, |
| PDE4B-216 | ENST00000531358.1 | 556 | No protein | Processed transcript | - | - | - | TSL:4, |
| PDE4B-218 | ENST00000534463.5 | 866 | No protein | Retained intron | - | - | - | TSL:3, |
| PDE4B-208 | ENST00000483863.2 | 572 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PDE4B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PDE4B had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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