CRISPR-U™ Gene Knockout Cell Line Strategy

NOL7 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NOL7 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NOL7
Gene id 51406
Organism Homo sapiens
Official full symbol nucleolar protein 7
Gene type protein-coding
Also known as C6orf90, PQBP3, RARG-1, dJ223E5.2
Summary The protein encoded by this gene localizes to the nucleolus, where it maintains nucleolar structure and cell growth rates. The encoded protein also functions as a tumor suppressor and regulator of angiogenesis. The RB tumor suppressor gene recruits transcription factors to this gene and positively regulates its expression. Two transcript variants encoding the same protein have been found for this gene.
Genomic regions Chromosome 6
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NOL7-202 ENST00000451315.7 1683 257aa Protein coding CCDS4528 Q9UMY1-1 NM_016167.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NOL7-201 ENST00000420088.1 646 83aa Protein coding - H7C2B1 - CDS 5' incomplete, TSL:2,
NOL7-203 ENST00000474485.1 920 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NOL7
This KO Strategy loading
Red Cotton™ Notes Gene NOL7 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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