CRISPR-U™ Gene Knockout Cell Line
Strategy
NOL7 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NOL7 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NOL7 |
| Gene id | 51406 |
| Organism | Homo sapiens |
| Official full symbol | nucleolar protein 7 |
| Gene type | protein-coding |
| Also known as | C6orf90, PQBP3, RARG-1, dJ223E5.2 |
| Summary | The protein encoded by this gene localizes to the nucleolus, where it maintains nucleolar structure and cell growth rates. The encoded protein also functions as a tumor suppressor and regulator of angiogenesis. The RB tumor suppressor gene recruits transcription factors to this gene and positively regulates its expression. Two transcript variants encoding the same protein have been found for this gene. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NOL7-202 | ENST00000451315.7 | 1683 | 257aa | Protein coding | CCDS4528 | Q9UMY1-1 | NM_016167.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NOL7-201 | ENST00000420088.1 | 646 | 83aa | Protein coding | - | H7C2B1 | - | CDS 5' incomplete, TSL:2, |
| NOL7-203 | ENST00000474485.1 | 920 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NOL7 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NOL7 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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