CRISPR-U™ Gene Knockout Cell Line
Strategy
PDE3B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PDE3B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PDE3B |
| Gene id | 5140 |
| Organism | Homo sapiens |
| Official full symbol | phosphodiesterase 3B |
| Gene type | protein-coding |
| Also known as | HcGIP1, cGIPDE1 |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PDE3B-201 | ENST00000282096.9 | 5995 | 1112aa | Protein coding | CCDS7817 | Q13370-1 | NM_000922.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| PDE3B-202 | ENST00000455098.2 | 3665 | 1061aa | Protein coding | - | Q13370-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PDE3B-205 | ENST00000534317.1 | 5521 | No protein | Processed transcript | - | - | - | TSL:1, |
| PDE3B-204 | ENST00000532740.1 | 788 | No protein | Processed transcript | - | - | - | TSL:2, |
| PDE3B-203 | ENST00000525439.1 | 693 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PDE3B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PDE3B had been KO in mcf7 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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