CRISPR-U™ Gene Knockout Cell Line Strategy

PDE3B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PDE3B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PDE3B
Gene id 5140
Organism Homo sapiens
Official full symbol phosphodiesterase 3B
Gene type protein-coding
Also known as HcGIP1, cGIPDE1
Genomic regions Chromosome 11
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PDE3B-201 ENST00000282096.9 5995 1112aa Protein coding CCDS7817 Q13370-1 NM_000922.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
PDE3B-202 ENST00000455098.2 3665 1061aa Protein coding - Q13370-2 - TSL:1, GENCODE basic, APPRIS ALT2,
PDE3B-205 ENST00000534317.1 5521 No protein Processed transcript - - - TSL:1,
PDE3B-204 ENST00000532740.1 788 No protein Processed transcript - - - TSL:2,
PDE3B-203 ENST00000525439.1 693 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PDE3B
This KO Strategy loading
Red Cotton™ Notes Gene PDE3B had been KO in mcf7 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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