CRISPR-U™ Gene Knockout Cell Line Strategy

CSAD Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CSAD Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CSAD
Gene id 51380
Organism Homo sapiens
Official full symbol cysteine sulfinic acid decarboxylase
Gene type protein-coding
Also known as CSD, PCAP
Summary This gene encodes a member of the group 2 decarboxylase family. A similar protein in rodents plays a role in multiple biological processes as the rate-limiting enzyme in taurine biosynthesis, catalyzing the decarboxylation of cysteinesulfinate to hypotaurine. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Genomic regions Chromosome 12
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CSAD-201 ENST00000267085.8 2645 520aa Protein coding CCDS8848 Q9Y600-3 - TSL:1, GENCODE basic,
CSAD-208 ENST00000444623.6 2614 493aa Protein coding CCDS58235 Q9Y600-1 NM_001244705.2 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CSAD-209 ENST00000453446.6 1774 493aa Protein coding CCDS58235 Q9Y600-1 - TSL:1, GENCODE basic, APPRIS P1,
CSAD-204 ENST00000379850.7 2379 519aa Protein coding - J3KPG9 - CDS 5' incomplete, TSL:5,
CSAD-202 ENST00000379843.7 2086 267aa Protein coding - Q9Y602 - TSL:5, GENCODE basic,
CSAD-203 ENST00000379846.5 1962 346aa Protein coding - Q9Y600-2 - TSL:5, GENCODE basic,
CSAD-219 ENST00000498635.1 585 129aa Protein coding - H3BTP4 - CDS 3' incomplete, TSL:4,
CSAD-207 ENST00000437073.5 581 163aa Protein coding - E9PFW9 - CDS 3' incomplete, TSL:4,
CSAD-206 ENST00000424990.5 565 146aa Protein coding - C9JTU8 - CDS 3' incomplete, TSL:4,
CSAD-220 ENST00000548698.5 455 135aa Protein coding - F8VV11 - CDS 3' incomplete, TSL:5,
CSAD-213 ENST00000475890.5 2080 267aa Nonsense mediated decay - Q9Y602 - TSL:5,
CSAD-205 ENST00000424845.5 558 45aa Nonsense mediated decay - F8WCW3 - TSL:3,
CSAD-214 ENST00000483632.5 558 142aa Nonsense mediated decay - H3BQ59 - CDS 5' incomplete, TSL:4,
CSAD-218 ENST00000491654.1 578 No protein Processed transcript - - - TSL:5,
CSAD-210 ENST00000454442.5 534 No protein Processed transcript - - - TSL:4,
CSAD-217 ENST00000490923.5 861 No protein Retained intron - - - TSL:3,
CSAD-215 ENST00000485004.5 751 No protein Retained intron - - - TSL:3,
CSAD-216 ENST00000490589.5 612 No protein Retained intron - - - TSL:3,
CSAD-212 ENST00000472908.1 543 No protein Retained intron - - - TSL:4,
CSAD-211 ENST00000461392.1 425 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CSAD
This KO Strategy loading
Red Cotton™ Notes Gene CSAD had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×