CRISPR-U™ Gene Knockout Cell Line
Strategy
CSAD Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CSAD Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CSAD |
| Gene id | 51380 |
| Organism | Homo sapiens |
| Official full symbol | cysteine sulfinic acid decarboxylase |
| Gene type | protein-coding |
| Also known as | CSD, PCAP |
| Summary | This gene encodes a member of the group 2 decarboxylase family. A similar protein in rodents plays a role in multiple biological processes as the rate-limiting enzyme in taurine biosynthesis, catalyzing the decarboxylation of cysteinesulfinate to hypotaurine. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CSAD-201 | ENST00000267085.8 | 2645 | 520aa | Protein coding | CCDS8848 | Q9Y600-3 | - | TSL:1, GENCODE basic, |
| CSAD-208 | ENST00000444623.6 | 2614 | 493aa | Protein coding | CCDS58235 | Q9Y600-1 | NM_001244705.2 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CSAD-209 | ENST00000453446.6 | 1774 | 493aa | Protein coding | CCDS58235 | Q9Y600-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CSAD-204 | ENST00000379850.7 | 2379 | 519aa | Protein coding | - | J3KPG9 | - | CDS 5' incomplete, TSL:5, |
| CSAD-202 | ENST00000379843.7 | 2086 | 267aa | Protein coding | - | Q9Y602 | - | TSL:5, GENCODE basic, |
| CSAD-203 | ENST00000379846.5 | 1962 | 346aa | Protein coding | - | Q9Y600-2 | - | TSL:5, GENCODE basic, |
| CSAD-219 | ENST00000498635.1 | 585 | 129aa | Protein coding | - | H3BTP4 | - | CDS 3' incomplete, TSL:4, |
| CSAD-207 | ENST00000437073.5 | 581 | 163aa | Protein coding | - | E9PFW9 | - | CDS 3' incomplete, TSL:4, |
| CSAD-206 | ENST00000424990.5 | 565 | 146aa | Protein coding | - | C9JTU8 | - | CDS 3' incomplete, TSL:4, |
| CSAD-220 | ENST00000548698.5 | 455 | 135aa | Protein coding | - | F8VV11 | - | CDS 3' incomplete, TSL:5, |
| CSAD-213 | ENST00000475890.5 | 2080 | 267aa | Nonsense mediated decay | - | Q9Y602 | - | TSL:5, |
| CSAD-205 | ENST00000424845.5 | 558 | 45aa | Nonsense mediated decay | - | F8WCW3 | - | TSL:3, |
| CSAD-214 | ENST00000483632.5 | 558 | 142aa | Nonsense mediated decay | - | H3BQ59 | - | CDS 5' incomplete, TSL:4, |
| CSAD-218 | ENST00000491654.1 | 578 | No protein | Processed transcript | - | - | - | TSL:5, |
| CSAD-210 | ENST00000454442.5 | 534 | No protein | Processed transcript | - | - | - | TSL:4, |
| CSAD-217 | ENST00000490923.5 | 861 | No protein | Retained intron | - | - | - | TSL:3, |
| CSAD-215 | ENST00000485004.5 | 751 | No protein | Retained intron | - | - | - | TSL:3, |
| CSAD-216 | ENST00000490589.5 | 612 | No protein | Retained intron | - | - | - | TSL:3, |
| CSAD-212 | ENST00000472908.1 | 543 | No protein | Retained intron | - | - | - | TSL:4, |
| CSAD-211 | ENST00000461392.1 | 425 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CSAD |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CSAD had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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