CRISPR-U™ Gene Knockout Cell Line Strategy

POP5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human POP5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol POP5
Gene id 51367
Organism Homo sapiens
Official full symbol POP5 homolog, ribonuclease P/MRP subunit
Gene type protein-coding
Also known as HSPC004, RPP2, RPP20, hPop5
Genomic regions Chromosome 12
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
POP5-202 ENST00000357500.5 1086 163aa Protein coding CCDS9202 Q969H6-1 NM_015918.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
POP5-201 ENST00000341039.6 913 113aa Protein coding CCDS9203 Q969H6-2 - TSL:2, GENCODE basic,
POP5-208 ENST00000542776.5 821 No protein Processed transcript - - - TSL:2,
POP5-209 ENST00000543355.5 794 No protein Processed transcript - - - TSL:3,
POP5-205 ENST00000539716.1 718 No protein Retained intron - - - TSL:2,
POP5-206 ENST00000541834.1 653 No protein Retained intron - - - TSL:2,
POP5-204 ENST00000535245.1 608 No protein Retained intron - - - TSL:3,
POP5-207 ENST00000542568.1 578 No protein Retained intron - - - TSL:NA,
POP5-203 ENST00000511394.2 494 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene POP5
This KO Strategy loading
Red Cotton™ Notes Gene POP5 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
Ubigene Red Cotton Workflow

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