CRISPR-U™ Gene Knockout Cell Line
Strategy
PDCD1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PDCD1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PDCD1 |
| Gene id | 5133 |
| Organism | Homo sapiens |
| Official full symbol | programmed cell death 1 |
| Gene type | protein-coding |
| Also known as | CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1 |
| Summary | This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PDCD1-201 | ENST00000334409.10 | 2097 | 288aa | Protein coding | CCDS33428 | A0A0M3M0G7 Q15116 | NM_005018.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PDCD1-202 | ENST00000343705.3 | 366 | 122aa | Protein coding | - | H0Y2W6 | - | CDS 5' and 3' incomplete, TSL:1, |
| PDCD1-203 | ENST00000418831.1 | 1019 | 66aa | Nonsense mediated decay | - | E7ER21 | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PDCD1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PDCD1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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