CRISPR-U™ Gene Knockout Cell Line
Strategy
ERGIC2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ERGIC2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ERGIC2 |
| Gene id | 51290 |
| Organism | Homo sapiens |
| Official full symbol | ERGIC and golgi 2 |
| Gene type | protein-coding |
| Also known as | CDA14, Erv41, PTX1, cd002 |
| Summary | ERGIC2, or PTX1, is a ubiquitously expressed nuclear protein that is downregulated in prostate carcinoma (Kwok et al., 2001 ). |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ERGIC2-201 | ENST00000360150.9 | 5033 | 377aa | Protein coding | CCDS41765 | A0A024RB08 Q96RQ1 | NM_016570.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ERGIC2-212 | ENST00000611644.4 | 4961 | 386aa | Protein coding | - | A0A087WU02 | - | TSL:5, GENCODE basic, |
| ERGIC2-203 | ENST00000546839.5 | 1274 | 220aa | Protein coding | - | F8VPA6 | - | CDS 3' incomplete, TSL:5, |
| ERGIC2-209 | ENST00000551467.5 | 765 | 209aa | Protein coding | - | H0YI50 | - | CDS 5' incomplete, TSL:5, |
| ERGIC2-210 | ENST00000552132.5 | 695 | 175aa | Protein coding | - | F8VR88 | - | CDS 3' incomplete, TSL:3, |
| ERGIC2-211 | ENST00000552155.5 | 693 | 203aa | Protein coding | - | F8VUZ2 | - | CDS 3' incomplete, TSL:3, |
| ERGIC2-206 | ENST00000548909.5 | 645 | 163aa | Protein coding | - | H0YI58 | - | CDS 5' incomplete, TSL:5, |
| ERGIC2-208 | ENST00000550353.5 | 616 | 172aa | Protein coding | - | F8W0R1 | - | CDS 3' incomplete, TSL:5, |
| ERGIC2-205 | ENST00000548441.1 | 432 | 71aa | Protein coding | - | F8VRW9 | - | CDS 3' incomplete, TSL:3, |
| ERGIC2-207 | ENST00000549182.1 | 565 | 49aa | Nonsense mediated decay | - | H0YHS0 | - | CDS 5' incomplete, TSL:5, |
| ERGIC2-202 | ENST00000546509.5 | 308 | 57aa | Nonsense mediated decay | - | H0YHJ8 | - | CDS 5' incomplete, TSL:3, |
| ERGIC2-204 | ENST00000548098.1 | 318 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ERGIC2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ERGIC2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: