CRISPR-U™ Gene Knockout Cell Line
Strategy
COA4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human COA4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | COA4 |
| Gene id | 51287 |
| Organism | Homo sapiens |
| Official full symbol | cytochrome c oxidase assembly factor 4 homolog |
| Gene type | protein-coding |
| Also known as | CHCHD8, CMC3, E2IG2 |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| COA4-206 | ENST00000545127.1 | 922 | 87aa | Protein coding | CCDS8225 | Q9NYJ1-1 | - | TSL:2, GENCODE basic, APPRIS P1, |
| COA4-201 | ENST00000355693.5 | 818 | 87aa | Protein coding | CCDS8225 | Q9NYJ1-1 | NM_016565.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| COA4-202 | ENST00000537289.1 | 582 | 87aa | Protein coding | CCDS8225 | Q9NYJ1-1 | - | TSL:2, GENCODE basic, APPRIS P1, |
| COA4-204 | ENST00000541455.1 | 1038 | 96aa | Protein coding | - | Q9NYJ1-2 | - | TSL:5, GENCODE basic, |
| COA4-203 | ENST00000537581.1 | 566 | No protein | Processed transcript | - | - | - | TSL:4, |
| COA4-205 | ENST00000544575.1 | 449 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | COA4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene COA4 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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