CRISPR-U™ Gene Knockout Cell Line Strategy

GOLM1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GOLM1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GOLM1
Gene id 51280
Organism Homo sapiens
Official full symbol golgi membrane protein 1
Gene type protein-coding
Also known as C9orf155, GOLPH2, GP73, HEL46, PSEC0257, bA379P1.3
Summary The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene.
Genomic regions Chromosome 9
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GOLM1-203 ENST00000388712.7 3046 401aa Protein coding CCDS35054 Q8NBJ4-1 NM_016548.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GOLM1-202 ENST00000388711.7 3022 401aa Protein coding CCDS35054 Q8NBJ4-1 - TSL:1, GENCODE basic, APPRIS P1,
GOLM1-208 ENST00000486130.5 869 121aa Protein coding - C9JYM4 - CDS 3' incomplete, TSL:4,
GOLM1-205 ENST00000466178.1 506 80aa Protein coding - C9J941 - CDS 3' incomplete, TSL:4,
GOLM1-206 ENST00000470762.6 561 105aa Nonsense mediated decay - F8WAT9 - TSL:5,
GOLM1-201 ENST00000257504.10 794 No protein Processed transcript - - - TSL:5,
GOLM1-207 ENST00000472919.1 476 No protein Processed transcript - - - TSL:4,
GOLM1-204 ENST00000464314.1 697 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GOLM1
This KO Strategy loading
Red Cotton™ Notes Gene GOLM1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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