CRISPR-U™ Gene Knockout Cell Line
Strategy
GOLM1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GOLM1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GOLM1 |
| Gene id | 51280 |
| Organism | Homo sapiens |
| Official full symbol | golgi membrane protein 1 |
| Gene type | protein-coding |
| Also known as | C9orf155, GOLPH2, GP73, HEL46, PSEC0257, bA379P1.3 |
| Summary | The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GOLM1-203 | ENST00000388712.7 | 3046 | 401aa | Protein coding | CCDS35054 | Q8NBJ4-1 | NM_016548.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GOLM1-202 | ENST00000388711.7 | 3022 | 401aa | Protein coding | CCDS35054 | Q8NBJ4-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| GOLM1-208 | ENST00000486130.5 | 869 | 121aa | Protein coding | - | C9JYM4 | - | CDS 3' incomplete, TSL:4, |
| GOLM1-205 | ENST00000466178.1 | 506 | 80aa | Protein coding | - | C9J941 | - | CDS 3' incomplete, TSL:4, |
| GOLM1-206 | ENST00000470762.6 | 561 | 105aa | Nonsense mediated decay | - | F8WAT9 | - | TSL:5, |
| GOLM1-201 | ENST00000257504.10 | 794 | No protein | Processed transcript | - | - | - | TSL:5, |
| GOLM1-207 | ENST00000472919.1 | 476 | No protein | Processed transcript | - | - | - | TSL:4, |
| GOLM1-204 | ENST00000464314.1 | 697 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GOLM1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GOLM1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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