CRISPR-U™ Gene Knockout Cell Line
Strategy
CDK17 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CDK17 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CDK17 |
| Gene id | 5128 |
| Organism | Homo sapiens |
| Official full symbol | cyclin dependent kinase 17 |
| Gene type | protein-coding |
| Also known as | PCTAIRE2, PCTK2 |
| Summary | The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It has similarity to a rat protein that is thought to play a role in terminally differentiated neurons. Alternatively spliced transcript variants encoding different isoforms have been found. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CDK17-201 | ENST00000261211.8 | 4036 | 523aa | Protein coding | CCDS9061 | Q00537-1 | NM_002595.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CDK17-203 | ENST00000543119.6 | 2442 | 523aa | Protein coding | CCDS53819 | Q00537-2 | - | TSL:2, GENCODE basic, |
| CDK17-202 | ENST00000542666.5 | 1769 | 470aa | Protein coding | - | F5H6Z0 | - | TSL:2, GENCODE basic, |
| CDK17-209 | ENST00000551816.5 | 626 | 179aa | Protein coding | - | F8VQZ9 | - | CDS 3' incomplete, TSL:4, |
| CDK17-211 | ENST00000552496.5 | 583 | 161aa | Protein coding | - | F8VZY6 | - | CDS 3' incomplete, TSL:3, |
| CDK17-210 | ENST00000552262.1 | 566 | 88aa | Protein coding | - | F8VUX2 | - | CDS 3' incomplete, TSL:3, |
| CDK17-205 | ENST00000548734.1 | 556 | 129aa | Protein coding | - | F8W1S5 | - | CDS 3' incomplete, TSL:4, |
| CDK17-212 | ENST00000553042.1 | 569 | No protein | Processed transcript | - | - | - | TSL:4, |
| CDK17-207 | ENST00000550971.5 | 482 | No protein | Processed transcript | - | - | - | TSL:3, |
| CDK17-204 | ENST00000548422.1 | 892 | No protein | Retained intron | - | - | - | TSL:3, |
| CDK17-208 | ENST00000551484.1 | 575 | No protein | Retained intron | - | - | - | TSL:4, |
| CDK17-206 | ENST00000549899.1 | 506 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CDK17 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CDK17 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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