CRISPR-U™ Gene Knockout Cell Line
Strategy
CRIM1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CRIM1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CRIM1 |
| Gene id | 51232 |
| Organism | Homo sapiens |
| Official full symbol | cysteine rich transmembrane BMP regulator 1 |
| Gene type | protein-coding |
| Also known as | CRIM-1, S52 |
| Summary | This gene encodes a transmembrane protein containing six cysteine-rich repeat domains and an insulin-like growth factor-binding domain. The encoded protein may play a role in tissue development though interactions with members of the transforming growth factor beta family, such as bone morphogenetic proteins. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CRIM1-201 | ENST00000280527.7 | 6060 | 1036aa | Protein coding | CCDS1783 | Q9NZV1 | NM_016441.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CRIM1-202 | ENST00000413985.1 | 874 | 175aa | Protein coding | - | H7C1Z0 | - | CDS 5' incomplete, TSL:3, |
| CRIM1-204 | ENST00000428774.1 | 700 | 55aa | Protein coding | - | H7C458 | - | CDS 5' incomplete, TSL:3, |
| CRIM1-203 | ENST00000426856.1 | 667 | 222aa | Protein coding | - | H7C2T6 | - | CDS 5' and 3' incomplete, TSL:3, |
| CRIM1-205 | ENST00000473403.5 | 386 | No protein | Processed transcript | - | - | - | TSL:3, |
| CRIM1-206 | ENST00000477491.5 | 311 | No protein | Processed transcript | - | - | - | TSL:2, |
| CRIM1-208 | ENST00000497236.1 | 240 | No protein | Processed transcript | - | - | - | TSL:3, |
| CRIM1-207 | ENST00000481321.1 | 346 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CRIM1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CRIM1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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