CRISPR-U™ Gene Knockout Cell Line
Strategy
HERC5 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HERC5 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HERC5 |
| Gene id | 51191 |
| Organism | Homo sapiens |
| Official full symbol | HECT and RLD domain containing E3 ubiquitin protein ligase 5 |
| Gene type | protein-coding |
| Also known as | CEB1, CEBP1 |
| Summary | This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HERC5-201 | ENST00000264350.8 | 3511 | 1024aa | Protein coding | CCDS3630 | Q9UII4 | NM_016323.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HERC5-203 | ENST00000508159.1 | 2243 | 662aa | Protein coding | - | E9PBL0 | - | TSL:2, GENCODE basic, |
| HERC5-204 | ENST00000508695.1 | 543 | No protein | Processed transcript | - | - | - | TSL:4, |
| HERC5-205 | ENST00000510223.5 | 2925 | No protein | Retained intron | - | - | - | TSL:2, |
| HERC5-202 | ENST00000502913.1 | 1949 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HERC5 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HERC5 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: