CRISPR-U™ Gene Knockout Cell Line Strategy

HERC5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HERC5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HERC5
Gene id 51191
Organism Homo sapiens
Official full symbol HECT and RLD domain containing E3 ubiquitin protein ligase 5
Gene type protein-coding
Also known as CEB1, CEBP1
Summary This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4.
Genomic regions Chromosome 4
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HERC5-201 ENST00000264350.8 3511 1024aa Protein coding CCDS3630 Q9UII4 NM_016323.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
HERC5-203 ENST00000508159.1 2243 662aa Protein coding - E9PBL0 - TSL:2, GENCODE basic,
HERC5-204 ENST00000508695.1 543 No protein Processed transcript - - - TSL:4,
HERC5-205 ENST00000510223.5 2925 No protein Retained intron - - - TSL:2,
HERC5-202 ENST00000502913.1 1949 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HERC5
This KO Strategy loading
Red Cotton™ Notes Gene HERC5 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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