CRISPR-U™ Gene Knockout Cell Line Strategy

PCNT Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PCNT Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PCNT
Gene id 5116
Organism Homo sapiens
Official full symbol pericentrin
Gene type protein-coding
Also known as KEN, MOPD2, PCN, PCNT2, PCNTB, PCTN2, SCKL4
Summary The protein encoded by this gene binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubule nucleation component gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression. Mutations in this gene cause Seckel syndrome-4 and microcephalic osteodysplastic primordial dwarfism type II. Two transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 21
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PCNT-201 ENST00000359568.10 10526 3336aa Protein coding CCDS33592 O95613-1 NM_006031.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PCNT-202 ENST00000418394.1 796 228aa Protein coding - H7C2A3 - CDS 5' incomplete, TSL:3,
PCNT-205 ENST00000480896.5 10485 No protein Processed transcript - - - TSL:1,
PCNT-208 ENST00000490468.5 1415 No protein Processed transcript - - - TSL:5,
PCNT-204 ENST00000466474.5 895 No protein Processed transcript - - - TSL:3,
PCNT-207 ENST00000483844.1 734 No protein Processed transcript - - - TSL:3,
PCNT-206 ENST00000482575.1 578 No protein Processed transcript - - - TSL:3,
PCNT-203 ENST00000465356.1 767 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PCNT
This KO Strategy loading
Red Cotton™ Notes Gene PCNT had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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