CRISPR-U™ Gene Knockout Cell Line
Strategy
CUTC Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CUTC Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CUTC |
| Gene id | 51076 |
| Organism | Homo sapiens |
| Official full symbol | cutC copper transporter |
| Gene type | protein-coding |
| Also known as | CGI-32 |
| Summary | Members of the CUT family of copper transporters are associated with copper homeostasis and are involved in the uptake, storage, delivery, and efflux of copper (Gupta et al., 1995 ; Li et al., 2005 ). |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CUTC-202 | ENST00000370476.10 | 1332 | 273aa | Protein coding | CCDS7483 | Q9NTM9 | NM_015960.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CUTC-201 | ENST00000370472.4 | 727 | 144aa | Protein coding | - | Q5TCZ7 | - | CDS 3' incomplete, TSL:5, |
| CUTC-203 | ENST00000471520.5 | 831 | 194aa | Nonsense mediated decay | - | A0A0B4J226 | - | TSL:1, |
| CUTC-204 | ENST00000493385.5 | 612 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CUTC |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CUTC had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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