CRISPR-U™ Gene Knockout Cell Line
Strategy
LAP3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LAP3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LAP3 |
| Gene id | 51056 |
| Organism | Homo sapiens |
| Official full symbol | leucine aminopeptidase 3 |
| Gene type | protein-coding |
| Also known as | HEL-S-106, LAP, LAPEP, PEPS |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LAP3-201 | ENST00000226299.9 | 2209 | 519aa | Protein coding | CCDS3422 | P28838-1 | NM_015907.3 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| LAP3-211 | ENST00000618908.4 | 2100 | 519aa | Protein coding | CCDS3422 | P28838-1 | - | TSL:1, GENCODE basic, APPRIS P2, |
| LAP3-210 | ENST00000606142.5 | 1876 | 488aa | Protein coding | - | P28838-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| LAP3-209 | ENST00000513105.1 | 626 | 208aa | Protein coding | - | H0Y9Q1 | - | CDS 5' and 3' incomplete, TSL:3, |
| LAP3-207 | ENST00000509583.1 | 887 | 119aa | Nonsense mediated decay | - | H0Y983 | - | CDS 5' incomplete, TSL:1, |
| LAP3-202 | ENST00000503467.1 | 1709 | No protein | Processed transcript | - | - | - | TSL:2, |
| LAP3-203 | ENST00000504614.5 | 813 | No protein | Processed transcript | - | - | - | TSL:3, |
| LAP3-204 | ENST00000507960.1 | 435 | No protein | Processed transcript | - | - | - | TSL:5, |
| LAP3-208 | ENST00000512397.1 | 689 | No protein | Retained intron | - | - | - | TSL:2, |
| LAP3-206 | ENST00000508497.5 | 651 | No protein | Retained intron | - | - | - | TSL:3, |
| LAP3-205 | ENST00000508014.5 | 494 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LAP3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LAP3 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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