CRISPR-U™ Gene Knockout Cell Line Strategy

LAP3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LAP3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LAP3
Gene id 51056
Organism Homo sapiens
Official full symbol leucine aminopeptidase 3
Gene type protein-coding
Also known as HEL-S-106, LAP, LAPEP, PEPS
Genomic regions Chromosome 4
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LAP3-201 ENST00000226299.9 2209 519aa Protein coding CCDS3422 P28838-1 NM_015907.3 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
LAP3-211 ENST00000618908.4 2100 519aa Protein coding CCDS3422 P28838-1 - TSL:1, GENCODE basic, APPRIS P2,
LAP3-210 ENST00000606142.5 1876 488aa Protein coding - P28838-2 - TSL:1, GENCODE basic, APPRIS ALT2,
LAP3-209 ENST00000513105.1 626 208aa Protein coding - H0Y9Q1 - CDS 5' and 3' incomplete, TSL:3,
LAP3-207 ENST00000509583.1 887 119aa Nonsense mediated decay - H0Y983 - CDS 5' incomplete, TSL:1,
LAP3-202 ENST00000503467.1 1709 No protein Processed transcript - - - TSL:2,
LAP3-203 ENST00000504614.5 813 No protein Processed transcript - - - TSL:3,
LAP3-204 ENST00000507960.1 435 No protein Processed transcript - - - TSL:5,
LAP3-208 ENST00000512397.1 689 No protein Retained intron - - - TSL:2,
LAP3-206 ENST00000508497.5 651 No protein Retained intron - - - TSL:3,
LAP3-205 ENST00000508014.5 494 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LAP3
This KO Strategy loading
Red Cotton™ Notes Gene LAP3 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×