CRISPR-U™ Gene Knockout Cell Line Strategy

GMNN Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GMNN Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GMNN
Gene id 51053
Organism Homo sapiens
Official full symbol geminin DNA replication inhibitor
Gene type protein-coding
Also known as Gem, MGORS6
Summary This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16.
Genomic regions Chromosome 6
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GMNN-201 ENST00000230056.8 1263 209aa Protein coding CCDS4560 A0A024QZY7 O75496 NM_015895.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GMNN-202 ENST00000356509.7 1133 209aa Protein coding CCDS4560 A0A024QZY7 O75496 - TSL:2, GENCODE basic, APPRIS P1,
GMNN-207 ENST00000620958.4 1039 209aa Protein coding CCDS4560 A0A024QZY7 O75496 - TSL:3, GENCODE basic, APPRIS P1,
GMNN-204 ENST00000378059.3 688 102aa Protein coding - H7C608 - CDS 3' incomplete, TSL:2,
GMNN-203 ENST00000378054.6 653 179aa Protein coding - E2QRF9 - CDS 3' incomplete, TSL:3,
GMNN-206 ENST00000476555.5 580 65aa Protein coding - C9K0U5 - CDS 3' incomplete, TSL:3,
GMNN-205 ENST00000468943.1 679 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GMNN
This KO Strategy loading
Red Cotton™ Notes Gene GMNN had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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