CRISPR-U™ Gene Knockout Cell Line
Strategy
GMNN Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GMNN Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GMNN |
| Gene id | 51053 |
| Organism | Homo sapiens |
| Official full symbol | geminin DNA replication inhibitor |
| Gene type | protein-coding |
| Also known as | Gem, MGORS6 |
| Summary | This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GMNN-201 | ENST00000230056.8 | 1263 | 209aa | Protein coding | CCDS4560 | A0A024QZY7 O75496 | NM_015895.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GMNN-202 | ENST00000356509.7 | 1133 | 209aa | Protein coding | CCDS4560 | A0A024QZY7 O75496 | - | TSL:2, GENCODE basic, APPRIS P1, |
| GMNN-207 | ENST00000620958.4 | 1039 | 209aa | Protein coding | CCDS4560 | A0A024QZY7 O75496 | - | TSL:3, GENCODE basic, APPRIS P1, |
| GMNN-204 | ENST00000378059.3 | 688 | 102aa | Protein coding | - | H7C608 | - | CDS 3' incomplete, TSL:2, |
| GMNN-203 | ENST00000378054.6 | 653 | 179aa | Protein coding | - | E2QRF9 | - | CDS 3' incomplete, TSL:3, |
| GMNN-206 | ENST00000476555.5 | 580 | 65aa | Protein coding | - | C9K0U5 | - | CDS 3' incomplete, TSL:3, |
| GMNN-205 | ENST00000468943.1 | 679 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GMNN |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GMNN had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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