CRISPR-U™ Gene Knockout Cell Line
Strategy
GLOD4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GLOD4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GLOD4 |
| Gene id | 51031 |
| Organism | Homo sapiens |
| Official full symbol | glyoxalase domain containing 4 |
| Gene type | protein-coding |
| Also known as | C17orf25, CGI-150, HC71 |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GLOD4-202 | ENST00000301329.10 | 2059 | 298aa | Protein coding | CCDS32520 | Q9HC38-2 | - | TSL:1, GENCODE basic, APPRIS P1, |
| GLOD4-203 | ENST00000536578.5 | 2497 | 502aa | Protein coding | - | F6TLX2 | - | TSL:5, GENCODE basic, |
| GLOD4-201 | ENST00000301328.9 | 1809 | 313aa | Protein coding | - | Q9HC38-1 | - | TSL:1, GENCODE basic, |
| GLOD4-215 | ENST00000576419.1 | 705 | 227aa | Protein coding | - | I3L3Q4 | - | CDS 3' incomplete, TSL:3, |
| GLOD4-206 | ENST00000573137.1 | 447 | 115aa | Protein coding | - | I3L1I0 | - | CDS 5' incomplete, TSL:3, |
| GLOD4-218 | ENST00000625892.1 | 111 | 36aa | Protein coding | - | I3NI24 | - | TSL:5, GENCODE basic, |
| GLOD4-219 | ENST00000628529.2 | 111 | 36aa | Protein coding | - | I3NI27 | - | TSL:5, GENCODE basic, |
| GLOD4-208 | ENST00000574554.5 | 1314 | 76aa | Nonsense mediated decay | - | I3L277 | - | TSL:2, |
| GLOD4-213 | ENST00000575851.5 | 1063 | 36aa | Nonsense mediated decay | - | I3NI24 | - | TSL:3, |
| GLOD4-211 | ENST00000575790.5 | 853 | 59aa | Nonsense mediated decay | - | I3L1F4 | - | TSL:5, |
| GLOD4-209 | ENST00000574581.5 | 677 | 52aa | Nonsense mediated decay | - | I3L2C2 | - | TSL:3, |
| GLOD4-210 | ENST00000575528.5 | 585 | 58aa | Nonsense mediated decay | - | I3L3G0 | - | CDS 5' incomplete, TSL:2, |
| GLOD4-216 | ENST00000576670.1 | 562 | 68aa | Nonsense mediated decay | - | K7ENF2 | - | CDS 5' incomplete, TSL:4, |
| GLOD4-205 | ENST00000572220.1 | 428 | 36aa | Nonsense mediated decay | - | I3NI27 | - | TSL:5, |
| GLOD4-212 | ENST00000575800.1 | 276 | No protein | Processed transcript | - | - | - | TSL:3, |
| GLOD4-214 | ENST00000576239.5 | 2737 | No protein | Retained intron | - | - | - | TSL:2, |
| GLOD4-204 | ENST00000571073.5 | 2311 | No protein | Retained intron | - | - | - | TSL:2, |
| GLOD4-207 | ENST00000574354.5 | 558 | No protein | Retained intron | - | - | - | TSL:4, |
| GLOD4-217 | ENST00000576750.1 | 558 | No protein | Retained intron | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GLOD4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GLOD4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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$49
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$599
Single gRNA lentivirus
Work flow
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