CRISPR-U™ Gene Knockout Cell Line Strategy

GLOD4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GLOD4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GLOD4
Gene id 51031
Organism Homo sapiens
Official full symbol glyoxalase domain containing 4
Gene type protein-coding
Also known as C17orf25, CGI-150, HC71
Genomic regions Chromosome 17
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GLOD4-202 ENST00000301329.10 2059 298aa Protein coding CCDS32520 Q9HC38-2 - TSL:1, GENCODE basic, APPRIS P1,
GLOD4-203 ENST00000536578.5 2497 502aa Protein coding - F6TLX2 - TSL:5, GENCODE basic,
GLOD4-201 ENST00000301328.9 1809 313aa Protein coding - Q9HC38-1 - TSL:1, GENCODE basic,
GLOD4-215 ENST00000576419.1 705 227aa Protein coding - I3L3Q4 - CDS 3' incomplete, TSL:3,
GLOD4-206 ENST00000573137.1 447 115aa Protein coding - I3L1I0 - CDS 5' incomplete, TSL:3,
GLOD4-218 ENST00000625892.1 111 36aa Protein coding - I3NI24 - TSL:5, GENCODE basic,
GLOD4-219 ENST00000628529.2 111 36aa Protein coding - I3NI27 - TSL:5, GENCODE basic,
GLOD4-208 ENST00000574554.5 1314 76aa Nonsense mediated decay - I3L277 - TSL:2,
GLOD4-213 ENST00000575851.5 1063 36aa Nonsense mediated decay - I3NI24 - TSL:3,
GLOD4-211 ENST00000575790.5 853 59aa Nonsense mediated decay - I3L1F4 - TSL:5,
GLOD4-209 ENST00000574581.5 677 52aa Nonsense mediated decay - I3L2C2 - TSL:3,
GLOD4-210 ENST00000575528.5 585 58aa Nonsense mediated decay - I3L3G0 - CDS 5' incomplete, TSL:2,
GLOD4-216 ENST00000576670.1 562 68aa Nonsense mediated decay - K7ENF2 - CDS 5' incomplete, TSL:4,
GLOD4-205 ENST00000572220.1 428 36aa Nonsense mediated decay - I3NI27 - TSL:5,
GLOD4-212 ENST00000575800.1 276 No protein Processed transcript - - - TSL:3,
GLOD4-214 ENST00000576239.5 2737 No protein Retained intron - - - TSL:2,
GLOD4-204 ENST00000571073.5 2311 No protein Retained intron - - - TSL:2,
GLOD4-207 ENST00000574354.5 558 No protein Retained intron - - - TSL:4,
GLOD4-217 ENST00000576750.1 558 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GLOD4
This KO Strategy loading
Red Cotton™ Notes Gene GLOD4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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