CRISPR-U™ Gene Knockout Cell Line Strategy

GLRX2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GLRX2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GLRX2
Gene id 51022
Organism Homo sapiens
Official full symbol glutaredoxin 2
Gene type protein-coding
Also known as CGI-133, GRX2
Summary The protein encoded by this gene is a member of the glutaredoxin family of proteins, which maintain cellular thiol homeostasis. These proteins are thiol-disulfide oxidoreductases that use a glutathione-binding site and one or two active cysteines in their active site. This gene undergoes alternative splicing to produce multiple isoforms, one of which is ubiquitously expressed and localizes to mitochondria, where it functions in mitochondrial redox homeostasis and is important for the protection against and recovery from oxidative stress. Other isoforms, which have more restrictive expression patterns, show cytosolic and nuclear localization, and are thought to function in cellular differentiation and transformation, possibly with a role in tumor progression.
Genomic regions Chromosome 1
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GLRX2-202 ENST00000367440.3 1131 165aa Protein coding CCDS1380 Q9NS18-2 - TSL:1, GENCODE basic, APPRIS ALT2,
GLRX2-201 ENST00000367439.8 682 164aa Protein coding CCDS1381 Q9NS18-1 NM_197962.3 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
GLRX2-204 ENST00000608166.2 5612 164aa Nonsense mediated decay CCDS1381 Q9NS18-1 - TSL:NA,
GLRX2-203 ENST00000472197.1 774 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GLRX2
This KO Strategy loading
Red Cotton™ Notes Gene GLRX2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×