CRISPR-U™ Gene Knockout Cell Line
Strategy
GLRX2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GLRX2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GLRX2 |
| Gene id | 51022 |
| Organism | Homo sapiens |
| Official full symbol | glutaredoxin 2 |
| Gene type | protein-coding |
| Also known as | CGI-133, GRX2 |
| Summary | The protein encoded by this gene is a member of the glutaredoxin family of proteins, which maintain cellular thiol homeostasis. These proteins are thiol-disulfide oxidoreductases that use a glutathione-binding site and one or two active cysteines in their active site. This gene undergoes alternative splicing to produce multiple isoforms, one of which is ubiquitously expressed and localizes to mitochondria, where it functions in mitochondrial redox homeostasis and is important for the protection against and recovery from oxidative stress. Other isoforms, which have more restrictive expression patterns, show cytosolic and nuclear localization, and are thought to function in cellular differentiation and transformation, possibly with a role in tumor progression. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GLRX2-202 | ENST00000367440.3 | 1131 | 165aa | Protein coding | CCDS1380 | Q9NS18-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| GLRX2-201 | ENST00000367439.8 | 682 | 164aa | Protein coding | CCDS1381 | Q9NS18-1 | NM_197962.3 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| GLRX2-204 | ENST00000608166.2 | 5612 | 164aa | Nonsense mediated decay | CCDS1381 | Q9NS18-1 | - | TSL:NA, |
| GLRX2-203 | ENST00000472197.1 | 774 | No protein | Processed transcript | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GLRX2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GLRX2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: