CRISPR-U™ Gene Knockout Cell Line Strategy

EXOSC1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human EXOSC1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol EXOSC1
Gene id 51013
Organism Homo sapiens
Official full symbol exosome component 1
Gene type protein-coding
Also known as CGI-108, CSL4, Csl4p, SKI4, Ski4p, p13
Summary This gene encodes a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening. The association of this protein with the exosome is mediated by protein-protein interactions with ribosomal RNA-processing protein 42 and ribosomal RNA-processing protein 46. Alternative splicing of this gene results in multiple transcript variants.
Genomic regions Chromosome 10
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
EXOSC1-204 ENST00000370902.8 1145 195aa Protein coding CCDS7459 Q9Y3B2 NM_016046.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
EXOSC1-202 ENST00000370885.8 718 170aa Protein coding CCDS81492 B1AMU3 - TSL:3, GENCODE basic,
EXOSC1-203 ENST00000370886.9 741 178aa Protein coding - B1AMU4 - TSL:5, GENCODE basic,
EXOSC1-210 ENST00000485122.6 564 139aa Protein coding - R4GNH9 - TSL:5, GENCODE basic,
EXOSC1-201 ENST00000370884.5 536 165aa Protein coding - B1AMU7 - CDS 5' incomplete, TSL:5,
EXOSC1-209 ENST00000477692.6 480 143aa Protein coding - R4GMQ7 - CDS 5' incomplete, TSL:5,
EXOSC1-205 ENST00000471049.5 642 No protein Processed transcript - - - TSL:5,
EXOSC1-206 ENST00000472345.5 451 No protein Processed transcript - - - TSL:3,
EXOSC1-207 ENST00000474309.1 260 No protein Processed transcript - - - TSL:5,
EXOSC1-211 ENST00000489158.1 2113 No protein Retained intron - - - TSL:2,
EXOSC1-208 ENST00000476234.5 739 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene EXOSC1
This KO Strategy loading
Red Cotton™ Notes Gene EXOSC1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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