CRISPR-U™ Gene Knockout Cell Line
Strategy
EXOSC1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EXOSC1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | EXOSC1 |
| Gene id | 51013 |
| Organism | Homo sapiens |
| Official full symbol | exosome component 1 |
| Gene type | protein-coding |
| Also known as | CGI-108, CSL4, Csl4p, SKI4, Ski4p, p13 |
| Summary | This gene encodes a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening. The association of this protein with the exosome is mediated by protein-protein interactions with ribosomal RNA-processing protein 42 and ribosomal RNA-processing protein 46. Alternative splicing of this gene results in multiple transcript variants. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| EXOSC1-204 | ENST00000370902.8 | 1145 | 195aa | Protein coding | CCDS7459 | Q9Y3B2 | NM_016046.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| EXOSC1-202 | ENST00000370885.8 | 718 | 170aa | Protein coding | CCDS81492 | B1AMU3 | - | TSL:3, GENCODE basic, |
| EXOSC1-203 | ENST00000370886.9 | 741 | 178aa | Protein coding | - | B1AMU4 | - | TSL:5, GENCODE basic, |
| EXOSC1-210 | ENST00000485122.6 | 564 | 139aa | Protein coding | - | R4GNH9 | - | TSL:5, GENCODE basic, |
| EXOSC1-201 | ENST00000370884.5 | 536 | 165aa | Protein coding | - | B1AMU7 | - | CDS 5' incomplete, TSL:5, |
| EXOSC1-209 | ENST00000477692.6 | 480 | 143aa | Protein coding | - | R4GMQ7 | - | CDS 5' incomplete, TSL:5, |
| EXOSC1-205 | ENST00000471049.5 | 642 | No protein | Processed transcript | - | - | - | TSL:5, |
| EXOSC1-206 | ENST00000472345.5 | 451 | No protein | Processed transcript | - | - | - | TSL:3, |
| EXOSC1-207 | ENST00000474309.1 | 260 | No protein | Processed transcript | - | - | - | TSL:5, |
| EXOSC1-211 | ENST00000489158.1 | 2113 | No protein | Retained intron | - | - | - | TSL:2, |
| EXOSC1-208 | ENST00000476234.5 | 739 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | EXOSC1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene EXOSC1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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