CRISPR-U™ Gene Knockout Cell Line
Strategy
PCBD1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PCBD1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PCBD1 |
| Gene id | 5092 |
| Organism | Homo sapiens |
| Official full symbol | pterin-4 alpha-carbinolamine dehydratase 1 |
| Gene type | protein-coding |
| Also known as | DCOH, PCBD, PCD, PHS |
| Summary | This gene encodes a member of the pterin-4-alpha-carbinolamine dehydratase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein functions as both a dehydratase involved in tetrahydrobiopterin biosynthesis, and as a cofactor for HNF1A-dependent transcription. A deficiency of this enzyme leads to hyperphenylalaninemia. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PCBD1-201 | ENST00000299299.4 | 787 | 104aa | Protein coding | CCDS31217 | P61457 | NM_000281.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PCBD1-202 | ENST00000493228.1 | 732 | No protein | Processed transcript | - | - | - | TSL:2, |
| PCBD1-203 | ENST00000493961.5 | 389 | No protein | Processed transcript | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PCBD1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PCBD1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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