CRISPR-U™ Gene Knockout Cell Line Strategy

PARD6A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PARD6A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PARD6A
Gene id 50855
Organism Homo sapiens
Official full symbol par-6 family cell polarity regulator alpha
Gene type protein-coding
Also known as PAR-6A, PAR6, PAR6C, PAR6alpha, TAX40, TIP-40
Summary This gene is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cell membrane protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. The protein also has a role in the epithelial-to-mesenchymal transition (EMT) that characterizes the invasive phenotype associated with metastatic carcinomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Genomic regions Chromosome 16
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PARD6A-201 ENST00000219255.3 1248 346aa Protein coding CCDS10843 Q9NPB6-1 - TSL:1, GENCODE basic, APPRIS P4,
PARD6A-202 ENST00000458121.7 1244 345aa Protein coding CCDS45514 Q9NPB6-2 NM_001037281.2 TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
PARD6A-203 ENST00000602551.5 1171 316aa Protein coding - R4GMM2 - TSL:5, GENCODE basic,
PARD6A-204 ENST00000602727.1 1281 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PARD6A
This KO Strategy loading
Red Cotton™ Notes Gene PARD6A had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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