CRISPR-U™ Gene Knockout Cell Line
Strategy
PARD6A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PARD6A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PARD6A |
| Gene id | 50855 |
| Organism | Homo sapiens |
| Official full symbol | par-6 family cell polarity regulator alpha |
| Gene type | protein-coding |
| Also known as | PAR-6A, PAR6, PAR6C, PAR6alpha, TAX40, TIP-40 |
| Summary | This gene is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cell membrane protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. The protein also has a role in the epithelial-to-mesenchymal transition (EMT) that characterizes the invasive phenotype associated with metastatic carcinomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PARD6A-201 | ENST00000219255.3 | 1248 | 346aa | Protein coding | CCDS10843 | Q9NPB6-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| PARD6A-202 | ENST00000458121.7 | 1244 | 345aa | Protein coding | CCDS45514 | Q9NPB6-2 | NM_001037281.2 | TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| PARD6A-203 | ENST00000602551.5 | 1171 | 316aa | Protein coding | - | R4GMM2 | - | TSL:5, GENCODE basic, |
| PARD6A-204 | ENST00000602727.1 | 1281 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PARD6A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PARD6A had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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