CRISPR-U™ Gene Knockout Cell Line
Strategy
ASAP1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ASAP1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ASAP1 |
| Gene id | 50807 |
| Organism | Homo sapiens |
| Official full symbol | ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 |
| Gene type | protein-coding |
| Also known as | AMAP1, CENTB4, DDEF1, PAG2, PAP, ZG14P |
| Summary | This gene encodes an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe involved in regulation of membrane trafficking and cytoskeleton remodeling. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ASAP1-201 | ENST00000357668.2 | 6344 | 1122aa | Protein coding | CCDS75788 | A0A0A0MRE5 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| ASAP1-202 | ENST00000518721.6 | 6259 | 1129aa | Protein coding | CCDS6362 | Q9ULH1-1 | NM_018482.4 | TSL:5, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| ASAP1-214 | ENST00000524124.5 | 5478 | 950aa | Protein coding | - | H0YBF7 | - | CDS 5' incomplete, TSL:1, |
| ASAP1-205 | ENST00000519483.5 | 1449 | 483aa | Protein coding | - | H0YBM4 | - | CDS 5' and 3' incomplete, TSL:5, |
| ASAP1-207 | ENST00000520342.5 | 566 | 33aa | Protein coding | - | E5RFD9 | - | CDS 3' incomplete, TSL:5, |
| ASAP1-212 | ENST00000521426.5 | 566 | 98aa | Protein coding | - | E5RHD7 | - | CDS 3' incomplete, TSL:4, |
| ASAP1-211 | ENST00000521075.5 | 5406 | 39aa | Nonsense mediated decay | - | E5RHA9 | - | TSL:1, |
| ASAP1-209 | ENST00000520927.5 | 742 | 39aa | Nonsense mediated decay | - | E5RHA9 | - | TSL:5, |
| ASAP1-215 | ENST00000524299.1 | 550 | 39aa | Nonsense mediated decay | - | E5RHA9 | - | TSL:4, |
| ASAP1-216 | ENST00000524367.5 | 962 | No protein | Processed transcript | - | - | - | TSL:5, |
| ASAP1-208 | ENST00000520625.1 | 606 | No protein | Processed transcript | - | - | - | TSL:3, |
| ASAP1-203 | ENST00000518957.1 | 446 | No protein | Processed transcript | - | - | - | TSL:3, |
| ASAP1-210 | ENST00000521057.1 | 720 | No protein | Retained intron | - | - | - | TSL:3, |
| ASAP1-204 | ENST00000519169.5 | 708 | No protein | Retained intron | - | - | - | TSL:2, |
| ASAP1-206 | ENST00000520189.1 | 572 | No protein | Retained intron | - | - | - | TSL:4, |
| ASAP1-213 | ENST00000524018.1 | 401 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ASAP1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ASAP1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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$49
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$599
Single gRNA lentivirus
Work flow
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