CRISPR-U™ Gene Knockout Cell Line Strategy

ASAP1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ASAP1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ASAP1
Gene id 50807
Organism Homo sapiens
Official full symbol ArfGAP with SH3 domain, ankyrin repeat and PH domain 1
Gene type protein-coding
Also known as AMAP1, CENTB4, DDEF1, PAG2, PAP, ZG14P
Summary This gene encodes an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe involved in regulation of membrane trafficking and cytoskeleton remodeling. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 8
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ASAP1-201 ENST00000357668.2 6344 1122aa Protein coding CCDS75788 A0A0A0MRE5 - TSL:5, GENCODE basic, APPRIS ALT2,
ASAP1-202 ENST00000518721.6 6259 1129aa Protein coding CCDS6362 Q9ULH1-1 NM_018482.4 TSL:5, GENCODE basic, APPRIS P4, MANE Select v0.92,
ASAP1-214 ENST00000524124.5 5478 950aa Protein coding - H0YBF7 - CDS 5' incomplete, TSL:1,
ASAP1-205 ENST00000519483.5 1449 483aa Protein coding - H0YBM4 - CDS 5' and 3' incomplete, TSL:5,
ASAP1-207 ENST00000520342.5 566 33aa Protein coding - E5RFD9 - CDS 3' incomplete, TSL:5,
ASAP1-212 ENST00000521426.5 566 98aa Protein coding - E5RHD7 - CDS 3' incomplete, TSL:4,
ASAP1-211 ENST00000521075.5 5406 39aa Nonsense mediated decay - E5RHA9 - TSL:1,
ASAP1-209 ENST00000520927.5 742 39aa Nonsense mediated decay - E5RHA9 - TSL:5,
ASAP1-215 ENST00000524299.1 550 39aa Nonsense mediated decay - E5RHA9 - TSL:4,
ASAP1-216 ENST00000524367.5 962 No protein Processed transcript - - - TSL:5,
ASAP1-208 ENST00000520625.1 606 No protein Processed transcript - - - TSL:3,
ASAP1-203 ENST00000518957.1 446 No protein Processed transcript - - - TSL:3,
ASAP1-210 ENST00000521057.1 720 No protein Retained intron - - - TSL:3,
ASAP1-204 ENST00000519169.5 708 No protein Retained intron - - - TSL:2,
ASAP1-206 ENST00000520189.1 572 No protein Retained intron - - - TSL:4,
ASAP1-213 ENST00000524018.1 401 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ASAP1
This KO Strategy loading
Red Cotton™ Notes Gene ASAP1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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